Macroporous resin purification of grass carp fish (Ctenopharyngodon idella) scale peptides with in vitro angiotensin-I converting enzyme (ACE) inhibitory ability
Macroporous resin purification of grass carp fish (Ctenopharyngodon idella) scale peptides with in vitro angiotensin-I converting enzyme (ACE) inhibitory ability
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DOI：
10.1016/j.foodchem.2009.04.015
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发表时间：
2009-12-01
期刊：
影响因子：
8.8
通讯作者：
Xu, Shiying
中科院分区：
文献类型：
--
作者：
Zhang, Fengxiang;Wang, Zhang;Xu, Shiying
研究了非极性大孔吸附树脂DA 201-C对草鱼鱼鳞肽的吸附动力学和热力学。FSPs的吸附受时间、pH和肽浓度的影响。吸附过程符合Langmuir吸附等温式，为吸热过程（Δ H < 43 kJ/mol）。DA 201-C对FSPs的吸附主要是疏水作用。动态吸附和梯度洗脱结果表明，DA 201-C树脂对疏水氨基酸含量较高的多肽具有良好的吸附富集能力，这些多肽在体外具有较高的ACE抑制活性。洗脱组分具有其原始ACE活性一半的最低浓度（IC_（50））为0.13mg/ml。结果表明，鱼鳞肽具有较好的体外ACE抑制活性，是一种良好的体外ACE抑制活性肽源。(C)2009爱思唯尔有限公司保留所有权利。
The adsorption dynamics and thermodynamics of grass carp fish scale peptides (FSPs) onto non-polar macroporous resins (MARs), DA201-C, have been investigated. The adsorption of FSPs was affected by time, pH and peptide concentration. The adsorption process followed the Langmuir adsorption isotherm, and was endothermic (Delta H < 43 kJ/mol). The predominant force in adsorption of FSPs onto DA201-C was hydrophobic. Depending on this force, the dynamic adsorption and gradient desorption results showed that DA201-C resins were good at desalting and enriching peptides with higher contents of hydrophobic amino acids, and these peptides had higher ACE inhibitory capabilities in vitro. The lowest concentration at which the eluted fraction possessed half of its original ACE activity (IC50) Was 0.13 mg/ml. The results indicated that fish scale peptides produced showed good ACE-inhibitory effect in vitro and fish scales are a good source of peptides with in vitro ACE inhibitory activity. (C) 2009 Elsevier Ltd. All rights reserved.