Continuous lipolysis in a reversed micellar membrane bioreactor
Continuous lipolysis in a reversed micellar membrane bioreactor
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反胶束膜生物反应器中的连续脂肪分解
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
J. Cabral
中科院分区：
文献类型：
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作者：
D. Prazeres;F. Garcia;J. Cabral
在连续反胶束膜生物反应器中，以AOT/异辛烷为溶剂，采用包埋的粘性色杆菌脂肪酶B对橄榄油进行酶解。在超滤组件中安装管状陶瓷膜，用于保留脂肪酶并将产物从反应介质中分离。将水填充的胶束与底物/溶剂溶液一起补充到反应器中以补偿反胶束的渗透。考察了底物浓度、停留时间和含水量对系统产率和转化率的影响。对于每种底物浓度，发现生产率和转化度之间存在线性关系。在长期操作中测试了生物反应器的操作稳定性，证实了该催化体系的高稳定性。
The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed micelles of AOT in isooctane was carried out in a continous reversed micellar membrane bioreactor. A tubular ceramic membrane installed in an ultrafiltration module was used to retain the lipase and separate the products from the reaction media. Water filled micelles were supplemented to the reactor together with the substrate/solvent solution to compensate for the permeation of reversed micelles. The influence of substrate concentration, residence time and water content in the productivity and conversion of the system were investigated. A linear relationship between productivity and conversion degree was found for each substrate concentration tested. Operational stability of the bioreactor was tested in a long term operation confirming the high stability of this catalytic system.