Role of oxidative stress in the pathogenesis of septic ileus in mice
Role of oxidative stress in the pathogenesis of septic ileus in mice
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DOI：
10.1111/j.1365-2982.2004.00618.x
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发表时间：
2005-04-01
影响因子：
3.5
通讯作者：
Pelckmans, PA
中科院分区：
文献类型：
--
作者：
De Winter, BY;Van Nassauw, L;Pelckmans, PA
我们研究了氧化应激在脓毒性肠梗阻发病机制中的作用。通过腹膜内（i. p.）腹腔注射脂多糖（LPS，20 μ g·kg ~（-1））。研究了两次腹腔注射超氧化物歧化酶[聚乙二醇（PEG）-SOD，4000 U kg（-1）]和过氧化氢酶（PEG-CAT，15000 U kg（-1））对胃排空、小肠转运和血浆总亚硝酸盐浓度的影响。我们还对胃和回肠组织进行了免疫组织化学实验。LPS显著延迟胃排空和肠转运，而血浆亚硝酸盐水平升高。聚乙二醇（PEG）-SOD逆转内毒素诱导的胃排空延迟，改善肠传输延迟，而不影响血浆亚硝酸盐水平。PEG-CAT轻微改善胃排空延迟，但对肠道传输无影响。免疫组化显示LPS处理小鼠胃和回肠粘膜中有硝基酪氨酸（NT）和4-羟基-2-壬烯醛（HNE）。用PEG-SOD或PEG-CAT处理LPS小鼠减少了两种组织中NT或HNE的存在。此外，LPS诱导诱导的诱导型一氧化氮合酶（iNOS）阳性的住宅巨噬细胞在胃和回肠的外部肌肉组织中的显着增加，PEG-SOD或PEG-CAT治疗后显着下降。本研究结果支持氧化和亚硝化应激在小鼠脓毒性肠梗阻发病机制中的作用。
We investigated the role of oxidative stress in the pathogenesis of septic ileus. Sepsis was induced by intraperitoneal (i.p.) injection of lipopolysaccharides (LPS, 20 ing kg(-1)) in mice. The effect of two i.p. injections of superoxide dismutase [polyethylene glycol (PEG)-SOD, 4000 U kg(-1)] and catalase (PEG-CAT, 15 000 U kg(-1)) was investigated on gastric emptying, intestinal transit and total nitrite plasma concentrations. We also performed immunohistochemical experiments on gastric and ilea] tissue. LPS significantly delayed gastric emptying and intestinal transit while plasma nitrite levels increased. Polyethylene glycol (PEG)-SOD reversed the endotoxin-induced delay in gastric emptying and improved the delay in intestinal transit without effect on plasma nitrite levels. PEG-CAT slightly improved the delay in gastric emptying without effect on intestinal transit. Irnmunohistochemistry showed the presence of nitrotyrosine (NT) and 4-hydroxy-2-nonenal (HNE) in the gastric and ileal mucosa of LPS-treated mice. Treatment with PEG-SOD or PEG-CAT of LPS mice diminished the presence of NT or HNE in both tissues. In addition, LPS induced a significant increase in inducible nitric oxide synthase (iNOS)-positive residential macrophages in the external musculature of stomach and ileum, which significantly decreased after PEG-SOD or PEG-CAT treatment. The present results support a role for oxidative and nitrosative stress in the pathogenesis of septic ileus in mice.