Impact of combined CO2 laser irradiation and fluoride on enamel and dentin biofilm-induced mineral loss
Impact of combined CO2 laser irradiation and fluoride on enamel and dentin biofilm-induced mineral loss
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DOI：
10.1007/s00784-016-1893-1
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发表时间：
2017-05-01
影响因子：
3.4
通讯作者：
Schwendicke, Falk
中科院分区：
文献类型：
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作者：
Esteves-Oliveira, Marcella;El-Sayed, Karim Fawzy;Schwendicke, Falk
用化学脱矿模型研究了CO2激光照射对牙釉质的防龋性。我们比较了CO2激光照射、氟化钠或两者同时照射对生物膜引起的矿物质丢失(匕首Z)和变形链球菌在牙釉质和牙本质上的粘附性的影响。将磨光的牛牙釉质和牙本质样本分为四组(n=12/组)：不处理组(C)；单独涂抹22,600-ppm氟化物(F)清漆(5%NaF)；单一CO2激光处理(L)和短脉冲(5亩S/波长=10.6 mU m)；以及激光和随后的氟化物处理(LF)。样本被消毒，并提交给自动化的单物种变形链球菌生物膜模型。脑心输液加5%蔗糖液，每日8次，然后用人工唾液冲洗。10天后，以菌落形成单位/毫升(cfu/ml)计数生物膜中的细菌数(n=7/组)。每组12人，用横断式显微摄影对匕首Z进行评估。统计分析采用单因素方差分析和后处理的显著差异检验，发现牙本质上的细菌数量显著高于牙釉质上的细菌数量(p<；0.01/ANOVA)。在牙本质上，LF组的CFU显著低于其他组(p=0.03/Tukey)，而牙釉质的CFU组之间没有差异。牙釉质中a匕首Z最低的是L(Mean/SD2036/1353vol%xmum)，不仅显著低于C(9642/2452vol%xmum)和F(7713/1489vol%xmum)(p<；0.05)，而且与LF(3135/2628vol%xmum)无显著差异(p>；0.05)。在牙本质中，只有LF(163/227)显著减少了匕首Z(p<；0.05)。在体外，CO2激光照射没有增加变形链球菌的粘附力。单独的激光治疗可以保护牙釉质免受生物膜诱导的脱矿，而联合应用激光和氟化物则需要保护牙本质。
The caries-protective effects of CO2 laser irradiation on dental enamel have been demonstrated using chemical demineralization models. We compared the effect of CO2 laser irradiation, sodium fluoride, or both on biofilm-induced mineral loss (a dagger Z) and Streptococcus mutans adhesion to enamel and dentin in vitro.Ground, polished bovine enamel, and dentin samples were allocated to four groups (n = 12/group): no treatment (C); single 22,600-ppm fluoride (F) varnish (5 % NaF) application; single CO2 laser treatment (L) with short pulses (5 mu s/lambda = 10.6 mu m); and laser and subsequent fluoride treatment (LF). Samples were sterilized and submitted to an automated mono-species S. mutans biofilm model. Brain heart infusion plus 5 % sucrose medium was provided eight times daily, followed by rinses with artificial saliva. After 10 days, bacterial numbers in biofilms were enumerated as colony-forming units/ml (CFU/ml) (n = 7/group). a dagger Z was assessed using transversal microradiography (n = 12/group). Univariate ANOVA with post hoc Tukey honestly-significant-difference test was used for statistical analysis.Bacterial numbers were significantly higher on dentin than enamel (p < 0.01/ANOVA). On dentin, LF yielded significantly lower CFUs than other groups (p = 0.03/Tukey), while no differences between groups were found for enamel. The lowest a dagger Z in enamel was observed for L (mean/SD 2036/1353 vol%x mu m), which was not only significantly lower than C (9642/2452 vol%x mu m) and F (7713/1489 vol%x mu m) (p < 0.05) but also not significantly different from LF (3135/2628 vol%x mu m) (p > 0.05). In dentin, only LF (163/227) significantly reduced a dagger Z (p < 0.05).CO2 laser irradiation did not increase adhesion of S. mutans in vitro. Laser treatment alone protected enamel against biofilm-induced demineralization, while a combined laser-fluoride application was required to protect dentin.