Two-dimensional electrophoresis analysis of differential protein expression in squamous carcinoma of the cervix
Two-dimensional electrophoresis analysis of differential protein expression in squamous carcinoma of the cervix
复制标题
宫颈鳞癌差异蛋白表达的二维电泳分析
DOI：
10.1007/s11670-008-0164-3
复制
发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
胡越
中科院分区：
文献类型：
--
作者：
邹双微;余丽蓉;吕杰强;朱雪琼;林毅;吴洁丽;胡越
ObjectiveTo establish and optimize the two-dimensional gel electrophoresis (2-DE) maps of squamous carcinoma of the cervix and to study the protein difference between squamous carcinoma of the cervix (SCC) and normal cervical tissue.MethodsUsing Two-dimensional gel electrophoresis followed by computer-assisted image analysis, the differential proteins between squamous carcinoma of the cervical tissue and normal cervical tissue were compared. Then using matrix-assisted laser desorption/ionization-time of flight mass spectrometry, the differential proteins were identified.ResultsThe well-resolved and reproducible two-dimensional gel electrophoresis patterns of squamous carcinoma of the cervix tissue and normal cervical tissue were obtained. After silver staining, the average matching ratio of squamous carcinoma of the cervix was 86.1%. There was a good reproducibility of spot position in 2-DE map, with average deviation in IEF direction of 0.95±0.13 mm, while in SDS-PAGE direction it was 1.20±0.18 mm. Ten protein spots were identified by mass spectrometry, some of which were involved in cell proliferation, cell apoptosis, intracellular enzymes, structural proteins, cycle regulation, and tumor occurrence.ConclusionThe differentially expressed proteins provide a fundamental basis for further study of human squamous carcinoma of the cervix and screening of its specific markers.
登录
查看更多内容
影响因子：
9.7
作者：
Llorca, Javier;Rodriguez-Cundin, Paz;Prieto-Salceda, Dolores
通讯作者：
Prieto-Salceda, Dolores
DOI：
10.1016/j.bpobgyn.2005.02.003
发表时间：
2005-08-01
影响因子：
5.5
作者：
Tiltman, AJ
通讯作者：
Tiltman, AJ
DOI：
10.1177/107327480701400406
发表时间：
2007-10
期刊：
Cancer control : journal of the Moffitt Cancer Center
影响因子：
--
作者：
C. Laronga;R. Drake
通讯作者：
C. Laronga;R. Drake
影响因子：
3.1
作者：
M. B. Watson;M. Lind;Laura Smith;P. Drew;L. Cawkwell
通讯作者：
M. B. Watson;M. Lind;Laura Smith;P. Drew;L. Cawkwell
影响因子：
6.6
作者：
D. Fenyö;R. Beavis
通讯作者：
D. Fenyö;R. Beavis