Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers.
Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers.
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由聚己内酯和聚二氧环己酮纤维组成的小直径混合血管移植物
DOI：
10.1038/s41598-017-03851-1
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发表时间：
2017-06-15
影响因子：
4.6
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Pan Y;Zhou X;Wei Y;Zhang Q;Wang T;Zhu M;Li W;Huang R;Liu R;Chen J;Fan G;Wang K;Kong D;Zhao Q
电纺聚己内酯(PCL)血管移植物具有良好的力学性能和通畅性。然而，PCL的缓慢降解限制了移植物中的血管再生。聚二氧杂环己酮(PDS)是一种可生物降解的聚合物，具有较高的机械强度和适中的体内降解率。本研究通过共电纺PCL和PDS纤维制备了一种小直径杂化血管移植物。与PCL接枝相比，PDS的加入改善了杂化接枝材料的力学性能和亲水性。体外/活体降解实验表明，PDS纤维在12周内完全降解，导致PCL/PDS接枝物的孔径增大。通过在大鼠腹主动脉置换模型上植入1个月和3个月来评价复合移植物的愈合特性。彩色多普勒超声显示PCL/PDS移植物通畅良好，未见动脉瘤样扩张。免疫荧光染色显示，由于表面亲水性的改善，PCL/PDS移植物的内皮细胞(ECs)覆盖率显著提高。PDS纤维的降解提供了额外的空间，促进了PCL/PDS移植物内血管平滑肌的再生。这些结果表明，PCL/PDS混合移植物可能是一种有前途的小直径血管移植物。
Electrospun polycaprolactone (PCL) vascular grafts showed good mechanical properties and patency. However, the slow degradation of PCL limited vascular regeneration in the graft. Polydioxanone (PDS) is a biodegradable polymer with high mechanical strength and moderate degradation ratein vivo. In this study, a small-diameter hybrid vascular graft was prepared by co-electrospinning PCL and PDS fibers. The incorporation of PDS improves mechanical properties, hydrophilicity of the hybrid grafts compared to PCL grafts. Thein vitro/vivodegradation assay showed that PDS fibers completely degraded within 12 weeks, which resulted in the increased pore size of PCL/PDS grafts. The healing characteristics of the hybrid grafts were evaluated by implantation in rat abdominal aorta replacement model for 1 and 3 months. Color Doppler ultrasound demonstrated PCL/PDS grafts had good patency, and did not show aneurysmal dilatation. Immunofluorescence staining showed the coverage of endothelial cells (ECs) was significantly enhanced in PCL/PDS grafts due to the improved surface hydrophilicity. The degradation of PDS fibers provided extra space, which facilitated vascular smooth muscle regeneration within PCL/PDS grafts. These results suggest that the hybrid PCL/PDS graft may be a promising candidate for the small-diameter vascular grafts.