Osteoclast precursors circulate in avian blood.
Osteoclast precursors circulate in avian blood.
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破骨细胞前体在禽类血液中循环。
DOI：
10.1007/bf00296217
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发表时间：
1992
影响因子：
4.2
通讯作者：
Teitelbaum,SL
中科院分区：
文献类型：
--
作者：
Alvarez,JI;Ross,FP;Athanasou,NA;Blair,HC;Greenfield,EM;Teitelbaum,SL
众所周知，破骨细胞来源于一种单核吞噬细胞家族的骨髓前体细胞，但这种细胞从骨髓转移到骨骼的方式尚不清楚。我们在此证明，单核祖细胞能够在体外分化成在鸡体内循环的具有破骨细胞表型的细胞。用密度梯度法从缺钙蛋鸡的血液中分离出单核部分，获得塑料粘附群体。这些细胞是单核吞噬细胞家族的成员，通过非特异性酯酶和抗酒石酸酸性磷酸酶（TRAP）活性、巨噬细胞特异性甘露糖受体的表达以及它们吞噬乳胶颗粒的能力证明了这一点。当在失活骨中培养时，这些细胞经历进行性多核，最终与分离的破骨细胞和骨髓前体产生的破骨细胞基本无法区分。具体来说，血源性多核细胞呈trap阳性，表现出特征性的褶皱膜，并表达破骨细胞抗原121F和23C6。当放置在骨片上时，这些细胞来自典型的吸收“坑”。此外，当与3h -脯氨酸标记的骨一起培养时，血单核细胞生成的破骨细胞与来自骨髓的破骨细胞一样有效地动员基质。因此，破骨细胞前体在蛋鸡的血液中循环，并可以在体外诱导分化。
The osteoclast is known to be derived from a marrow-residing precursor that is a member of the mononuclear phagocyte family, but the means by which this cell moves from marrow to bone is unknown. We herein demonstrate that mononuclear progenitors capable of differentiating,in vitro, into cells exhibiting the osteoclast phenotype circulate in chickens. The mononuclear fraction was isolated on a density gradient from blood drawn from calcium-deprived laying hens and the plastic-adherent population was obtained. These cells are members of the mononuclear phagocyte family, as demonstrated by nonspecific esterase and tartrate-resistant acid phosphatase (TRAP) activities, expression of the macrophage-specific mannose receptor, and their ability to phagocytose latex particles. When cultured in the presence of devitalized bone, these cells undergo progressive multinucleation and ultimately become essentially indistinguishable from isolated osteoclasts and those generated from bone marrow precursors. Specifically, the blood-derived polykaryons are TRAP-positive, exhibit characteristic ruffled membranes, and express the osteoclast antigens 121F and 23C6. When placed on bone slices, these cells from typical resorptive “pits.” Moreover, when cultured with3H-proline-labeled bone, the blood monocytegenerated osteoclasts mobilize matrix as effectively as those derived from marrow. Thus, osteoclast precursors circulate in the blood of laying hens and can be induced to differentiatein vitro.