Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena
Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena
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DOI：
10.1038/ncomms5157
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发表时间：
2014-06
影响因子：
16.6
通讯作者：
Y. Takehana;M. Matsuda;T. Myosho;M. Suster;K. Kawakami;T. Shin-I;Y. Kohara;Y. Kuroki;A. Toyoda;A. Fujiyama;S. Hamaguchi;M. Sakaizumi;K. Naruse
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文献类型：
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作者：
Y. Takehana;M. Matsuda;T. Myosho;M. Suster;K. Kawakami;T. Shin-I;Y. Kohara;Y. Kuroki;A. Toyoda;A. Fujiyama;S. Hamaguchi;M. Sakaizumi;K. Naruse
性染色体携带着一个主要的性别决定信号，它触发了生物体的性发育。然而，不同的性染色体系统已经在脊椎动物中进化。在这里，我们使用位置克隆，以确定性别决定基因座的青鳉相关的鱼，Oryzias dancena，并发现该基因座的Y染色体上含有acis调控元件，上调neighboringSox 3表达在性腺发育。Sox 3 Y转基因鱼的性别逆转表型和Sox 3 Y功能丧失突变体都表明它在性别决定中的关键作用。此外，我们证明，Sox 3启动睾丸分化通过上调下游Gsdf的表达，这是高度保守的鱼类性别分化途径。我们的研究结果不仅提供了强有力的证据，独立招聘ofSox 3的男性决定在远亲脊椎动物，但也提供了直接的证据，一个新的性别决定途径已经演变通过co-option的转录调控可能与保守的下游组件相互作用。
Sex chromosomes harbour a primary sex-determining signal that triggers sexual development of the organism. However, diverse sex chromosome systems have been evolved in vertebrates. Here we use positional cloning to identify the sex-determining locus of a medaka-related fish,Oryzias dancena, and find that the locus on the Y chromosome contains acis-regulatory element that upregulates neighbouringSox3expression in developing gonad. Sex-reversed phenotypes inSox3Ytransgenic fish, andSox3Yloss-of-function mutants all point to its critical role in sex determination. Furthermore, we demonstrate thatSox3initiates testicular differentiation by upregulating expression of downstreamGsdf, which is highly conserved in fish sex differentiation pathways. Our results not only provide strong evidence for the independent recruitment ofSox3to male determination in distantly related vertebrates, but also provide direct evidence that a novel sex determination pathway has evolved through co-option of a transcriptional regulator potentially interacted with a conserved downstream component.