Autoclaved sludge as the ideal seed to culture anammox bacteria: Reactor performance and microbial community diversity
Autoclaved sludge as the ideal seed to culture anammox bacteria: Reactor performance and microbial community diversity
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蒸压污泥作为培养厌氧氨氧化菌的理想种子：反应器性能和微生物群落多样性
DOI：
10.1016/j.biortech.2017.07.161
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发表时间：
2017
影响因子：
11.4
通讯作者：
Ni Shou-Qing
中科院分区：
文献类型：
--
作者：
Wang Yu;Bu Cui-Na;Kang Qi;Ahmad Hafiz Adeel;Zhang Jian;Gao Baoyu;Ni Shou-Qing
降低接种物中厌氧氨氧化菌的活性，可促进厌氧氨氧化菌的增殖和厌氧氨氧化工艺的实现。以0.3%厌氧氨氧化活性污泥为接种物，采用高压灭菌污泥（经高压灭菌预处理的厌氧颗粒污泥）成功实现了厌氧氨氧化工艺在UASB反应器中的快速启动。连续试验表明，R2（压蒸污泥投加）可将启动周期从72 d缩短到63 d。使用q-PCR测定，R1（对照）和R2的前50天厌氧氨氧化菌种群比生长率（μ）分别为0.014 d− 1和0.045 d− 1。对96-225 d脱氮性能的变异系数分析表明，R2比R1更稳定。Illumina MiSeq测序结果表明，高压灭菌可降低污泥的微生物多样性，提高厌氧氨氧化菌的丰度。此外，PICRUSt群落功能预测和c-di-GMP测定结果表明，R2具有较高的稳定性。
Reducing activity of commensal bacteria in inocula may enhance anammox bacteria proliferation and realization of anammox process. Fast start-up of anammox process in an UASB reactor was successfully achieved by using autoclaved sludge (anaerobic granular sludge pretreated by autoclaving) and 0.3% active anammox sludge as inoculum. Continuous experiments indicated that R2 (autoclaved sludge addition) could shorten the start-up period from 72 days to 63 days. The first 50 days anammox population specific growth rates (μ) of R1 (the control) and R2 were determined to be 0.014 d−1and 0.045 d−1using q-PCR assays. Analysis of coefficient of variations of nitrogen removal performance during days 96–225 indicated that R2 was more stable than R1. The Illumina MiSeq sequencing showed that autoclaving could decrease microbial diversity of sludge and enhance the abundance of anammox bacteria. Furthermore, PICRUSt community functions forecast and c-di-GMP measure illuminated the result of higher stability in R2.