Molecular characterization of mutant mouse strains generated from the EUCOMM/KOMP-CSD ES cell resource.
Molecular characterization of mutant mouse strains generated from the EUCOMM/KOMP-CSD ES cell resource.
复制标题
DOI：
10.1007/s00335-013-9467-x
复制
发表时间：
2013-08
期刊：
影响因子：
2.5
通讯作者：
Ramirez-Solis, Ramiro
中科院分区：
文献类型：
--
作者：
Ryder, Edward;Gleeson, Diane;Sethi, Debarati;Vyas, Sapna;Miklejewska, Evelina;Dalvi, Priya;Habib, Bishoy;Cook, Ross;Hardy, Matthew;Jhaveri, Kalpesh;Bottomley, Joanna;Wardle-Jones, Hannah;Bussell, James N.;Houghton, Richard;Salisbury, Jennifer;Skarnes, William C.;Ramirez-Solis, Ramiro
桑格小鼠遗传学项目使用EUCOMM/KOMP-CSD胚胎干（ES）细胞集产生敲除小鼠品系，并使用高通量初步表型筛选表征突变的后果。实现种系传播后，新菌株将接受一组基于PCR和qPCR的质量控制（QC）检测，以确认正确的靶向、盒结构和3 'LoxP位点的存在（需要潜在的条件性）。等位基因）。我们报告说，在研究的731株菌株中，超过86%的菌株显示出正确的靶向和盒结构，其中97%保留了3′ LoxP位点。我们讨论了未通过QC的细胞系的特征，并假设其中大多数可能是由于混合的ES细胞群，这些细胞群在创建ES细胞资源时采用的原始筛选技术无法检测到。本文的在线版本（doi：10.1007/s 00335 -013-9467-x）包含补充材料，可供授权用户使用。
The Sanger Mouse Genetics Project generates knockout mice strains using the EUCOMM/KOMP-CSD embryonic stem (ES) cell collection and characterizes the consequences of the mutations using a high-throughput primary phenotyping screen. Upon achieving germline transmission, new strains are subject to a panel of quality control (QC) PCR- and qPCR-based assays to confirm the correct targeting, cassette structure, and the presence of the 3′ LoxP site (required for the potential conditionality of the allele). We report that over 86 % of the 731 strains studied showed the correct targeting and cassette structure, of which 97 % retained the 3′ LoxP site. We discuss the characteristics of the lines that failed QC and postulate that the majority of these may be due to mixed ES cell populations which were not detectable with the original screening techniques employed when creating the ES cell resource. The online version of this article (doi:10.1007/s00335-013-9467-x) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
46.9
作者：
Prosser, Haydn M.;Koike-Yusa, Hiroko;Cooper, James D.;Law, Frances C.;Bradley, Allan
通讯作者：
Bradley, Allan
影响因子：
64.8
作者：
Skarnes WC;Rosen B;West AP;Koutsourakis M;Bushell W;Iyer V;Mujica AO;Thomas M;Harrow J;Cox T;Jackson D;Severin J;Biggs P;Fu J;Nefedov M;de Jong PJ;Stewart AF;Bradley A
通讯作者：
Bradley A
影响因子：
9.8
作者：
Church DM;Goodstadt L;Hillier LW;Zody MC;Goldstein S;She X;Bult CJ;Agarwala R;Cherry JL;DiCuccio M;Hlavina W;Kapustin Y;Meric P;Maglott D;Birtle Z;Marques AC;Graves T;Zhou S;Teague B;Potamousis K;Churas C;Place M;Herschleb J;Runnheim R;Forrest D;Amos-Landgraf J;Schwartz DC;Cheng Z;Lindblad-Toh K;Eichler EE;Ponting CP;Mouse Genome Sequencing Consortium
通讯作者：
Mouse Genome Sequencing Consortium
影响因子：
5.3
作者：
Duan, CJ;Yang, HY;Rui, LY
通讯作者：
Rui, LY
DOI：
10.1073/pnas.0337561100
发表时间：
2003-02-04
影响因子：
11.1
作者：
Guigó, R;Dermitzakis, ET;Brent, MR
通讯作者：
Brent, MR