In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice
In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice
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DOI：
10.1002/jor.20047
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Lee, OK
中科院分区：
文献类型：
--
作者：
Chou, SH;Kuo, TK;Lee, OK
间充质干细胞（MSC）是可以从人骨髓中分离出来的多能细胞，具有分化为胚胎中胚层后代的潜力。然而，目前的证据主要基于体外实验。我们使用小鼠子宫内移植（IUT）模型来研究人类 MSC 的植入能力。通过阴性免疫选择和有限稀释从骨髓中获得间充质干细胞，并通过流式细胞术和体外分化为成骨细胞、软骨细胞和脂肪细胞来表征。 MSC 被移植到胎龄 14 天的小鼠胎儿体内。通过流式细胞术、聚合酶链反应和荧光原位杂交 (FISH) 测定人 MSC 的植入。 MSC 被移植到源自所有三个胚层的组织中，并在分娩后持续存在长达 4 个月或更长时间，人类特异性 β-2 微球蛋白基因的表达和供体来源细胞的 FISH 证明了这一点。在受者的外周血中可检测到供体来源的CD45(+)细胞，表明间充质干细胞在胎儿阶段参与造血作用。该模型可以进一步用于评估 MSC 的可能应用。 (c) 2006 年骨科研究学会。由 Wiley 期刊公司出版
Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from human bone marrow and possess the potential to differentiate into progenies of embryonic mesoderm. However, current evidence is based predominantly on in vitro experiments. We used a murine model of in utero transplantation (IUT) to study the engraftment capabilities of human MSCs. MSCs were obtained from bone marrow by negative immunoselection and limiting dilution, and were characterized by flow cytometry and by in vitro differentiation into osteoblasts, chondrocytes, and adipocytes. MSCs were transplanted into fetal mice at a gestational age of 14 days. Engraftment of human MSCs was determined by flow cytometry, polymerase chain reaction, and fluorescence in situ hybridization (FISH). MSCs engrafted into tissues originating from all three germ layers and persisted for up to 4 months or more after delivery, as evidenced by the expression of the human-specific beta-2 microglobulin gene and by FISH for donor-derived cells. Donor-derived CD45(+) cells were detectable in the peripheral blood of recipients, suggesting the participation of MSCs in hematopoiesis at the fetal stage. This model can further serve to evaluate possible applications of MSCs. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.