Environmental controls and composition of anoxygenic photoheterotrophs in ultraoligotrophic high- altitude lakes ( Central Pyrenees)
Environmental controls and composition of anoxygenic photoheterotrophs in ultraoligotrophic high- altitude lakes ( Central Pyrenees)
复制标题
DOI：
10.1111/1758-2229.12142
复制
发表时间：
2014-04-01
影响因子：
3.3
通讯作者：
Casamayor, Emilio O.
中科院分区：
文献类型：
--
作者：
Caliz, Joan;Casamayor, Emilio O.
淡水厌氧光异养生物(AP)的系统发育组成与它们的海洋对应生物相比一直没有得到很好的研究。在本研究中，我们利用pufM基因变性梯度凝胶电泳指纹图谱，并对选定的湖泊样品进行克隆和测序，探索了一组超寡营养的寒冷高山湖泊(西班牙中部比利牛斯山)。探讨了不同范围的湖湖学和物理化学值作为AP丰度和组成的环境驱动因素。我们没有观察到水体丰富度/多样性与所测量的任何湖沼特征或营养状况之间的显著关系，而与氨浓度呈负相关。电导率、pH和硝酸盐浓度与AP群落组成的变化显著相关，而湖泊面积、海拔、温度和营养状况与其变化关系不显著。大多数序列(~GT；SulfitobacterandBrevundimonas(Alphaproteobacteria)，)与数据库中最近的同源序列(贝塔变形杆菌)最接近，而数量较少的克隆与红杆菌的亲缘关系较近，与之前在area.Congregibacter‐likeGammaproteobacteriawere中发现的未检测到的16S 基因序列一致。与内陆水域的现有研究相比，分类上沿盐度梯度进行了划分，大陆水体中的类康氏杆菌序列仅限于高盐度条件下。
The phylogenetic composition of freshwater anoxygenic photoheterotrophs (APs) has been poorly investigated as compared with their marine counterparts. In this study, we explored a set of ultraoligotrophic cold high mountain lakes (Central Pyrenees, Spain) by bothpufMgene denaturing gradient gel electrophoresis fingerprinting, and cloning and sequencing of selected lakes samples. Different ranges of limnological and physico‐chemical values were explored as environmental drivers of APs richness and composition. We did not observe significant relationships between richness/diversity ofpufMand any of the limnological characteristics measured or trophic status, but a negative correlation with ammonia concentration. Conductivity, pH and nitrate concentration were significantly related to changes in APs community composition, whereas lake area, altitude, temperature and trophic status did not. Most of the sequences (> 85%) had thepufMsequences ofLimnohabitans(Betaproteobacteria)as the closest relative in databases, whereas less abundant clones were more closely related toRhodobacter,SulfitobacterandBrevundimonas(Alphaproteobacteria), in agreement with 16S rRNA gene sequences previously found in the area.Congregibacter‐likeGammaproteobacteriawere not detected. Comparison with available studies in inland waters showed taxonomic partitioning along salinity gradients, andCongregibacter‐like sequences restricted to high saline conditions in continental water bodies.