Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt
Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt
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DOI：
10.1111/j.1348-0421.2012.00429.x
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发表时间：
2012-04-01
影响因子：
2.6
通讯作者：
Shimamoto, Tadashi
中科院分区：
文献类型：
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作者：
Ahmed, Ashraf M.;Shimamoto, Tadashi
迄今为止，还没有关于沙门氏菌耐药性分子基础的信息。从埃及甚至非洲的家禽中提取因此，本研究的目的是分析，在分子水平上，在埃及的患病肉鸡回收沙门氏菌菌株的多重耐药机制。鉴定出21株沙门氏菌分离株;其中13株为肠道沙门氏菌血清型肠伤寒和8株肠道沙门氏菌血清型鼠伤寒。17人（81%）。沙门氏菌分离株显示多重耐药表型，特别是对氨苄青霉素，链霉素，大观霉素，卡那霉素，四环素，氯霉素，和甲氧苄啶/磺胺甲恶唑。PCR和DNA测序鉴定出9株（42.9%）为1类整合子，3株（14.3%）为2类整合子。在1类整合子内鉴定的耐药基因为A型氨基糖苷腺苷酰转移酶aadA 1、aadA 2和aadA 5以及A型二氢叶酸还原酶dfrA 1、dfrA 5、dfrA 12、dfrA 15和dfrA 17。同时还鉴定了β-内酰胺酶编码基因blaTEM-1和blaCMY-2以及氟苯尼考耐药基因floR。14株（66.7%）沙门菌中检出四环素耐药基因泰特（A）。据我们所知，这是第一份关于沙门氏菌耐药性分子基础的报告。从非洲的家禽中分离出来。
To date, no information has been available on the molecular bases of antimicrobial resistance in Salmonella spp. from poultry in Egypt or even in Africa. Therefore, the objective of this study was to analyze, at the molecular level, the mechanisms of multidrug-resistance in isolates of Salmonella recovered from diseased broilers in Egypt. Twenty-one Salmonella isolates were identified; 13 of these isolates were Salmonella enterica serovar Enteritidis and eight Salmonella enterica serovar Typhimurium. 17 (81%). Salmonella isolates displayed multidrug resistance phenotypes, particularly against ampicillin, streptomycin, spectinomycin, kanamycin, tetracycline, chloramphenicol, and trimethoprim/sulfamethoxazole. PCR and DNA sequencing identified class 1 integrons in nine (42.9%) isolates and class 2 integrons in three (14.3%) isolates. The identified resistance genes within class 1 integrons were aminoglycoside adenyltransferase type A, aadA1, aadA2 and aadA5 and dihydrofolate reductase type A, dfrA1, dfrA5, dfrA12, dfrA15 and dfrA17. The beta-lactamase encoding genes blaTEM-1 and blaCMY-2 and florfenicol resistance gene floR were also identified. Furthermore, the tetracycline resistance gene tet(A) was identified in 14 (66.7%) Salmonella isolates. To the best of our knowledge, this is the first report of the molecular basis of antimicrobial resistance in Salmonella spp. isolated from poultry in Africa.