A Role for Codon Order in Translation Dynamics
A Role for Codon Order in Translation Dynamics
复制标题
DOI：
10.1016/j.cell.2010.02.036
复制
发表时间：
2010-04-16
期刊：
影响因子：
64.5
通讯作者：
Barral, Yves
中科院分区：
文献类型：
--
作者：
Cannarrozzi, Gina;Schraudolph, Nicol N.;Barral, Yves
遗传密码是退化的。每个氨基酸由多达六个同义密码子编码;这些密码子之间的选择影响基因表达。在这里，我们表明，在编码序列中，一旦使用了特定的密码子，随后出现的相同氨基酸不会随机使用密码子，但有利于使用相同的tRNA的密码子。该效应在快速诱导的基因中是显著的，涉及频繁和罕见的密码子，并且仅缓慢地作为后续同义密码子之间的距离的函数而减小。此外，我们发现，在S。酿酒酵母密码子相关性相对于同义但与酿酒酵母相关的序列的翻译加速翻译。这些数据表明，tRNA从核糖体扩散出去比翻译慢，并且一些tRNA通道发生在核糖体上。他们还确定翻译的动态在基因组水平上留下了重要的签名。
The genetic code is degenerate. Each amino acid is encoded by up to six synonymous codons; the choice between these codons influences gene expression. Here, we show that in coding sequences, once a particular codon has been used, subsequent occurrences of the same amino acid do not use codons randomly, but favor codons that use the same tRNA. The effect is pronounced in rapidly induced genes, involves both frequent and rare codons and diminishes only slowly as a function of the distance between subsequent synonymous codons. Furthermore, we found that in S. cerevisiae codon correlation accelerates translation relative to the translation of synonymous yet anticorrelated sequences. The data suggest that tRNA diffusion away from the ribosome is slower than translation, and that some tRNA channeling takes place at the ribosome. They also establish that the dynamics of translation leave a significant signature at the level of the genome.