Cholecystokinin and pancreozymin, one single hormone?
Cholecystokinin and pancreozymin, one single hormone?
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胆囊收缩素和胰酶，是一种激素吗？
DOI：
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发表时间：
1966
期刊：
影响因子：
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通讯作者：
V. Mutt
中科院分区：
文献类型：
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作者：
E. Jorpes;V. Mutt
促胰液素、胆囊收缩素（GCK）和胰酶素（PZ）被从热凝固的猪十二指肠-空肠粘膜的稀乙酸提取物中提取的海藻酸吸收。洗脱后，用氯化钠沉淀。将盐饼中的分泌素溶于甲醇中。对于不溶于甲醇且含有5个Ivy dog单位（IDU）CCK/mg的材料，CCK和PZ活性吸附在CMC纤维素上，活性材料在TEAE纤维素上进一步色谱分离，得到含有250个IDU CCK/mg的产品。通过Sephadex G 50柱和随后在Amberlite XE-64柱上层析来实现进一步纯化。活性现在为3，000（6，000）IDU/mg。由于哈珀和Raper的原始PZ制剂和Pancreozymin Boots的商业样品每mg含有0.3 Ivy dog单位的胆囊收缩素，胆囊收缩素活性已富集10，000-（20，000-）倍。在豚鼠中测定的CCK活性强度增加的同时，在猫中测定的PZ活性在三种不同强度水平（即0.3）下也同样增加。220和3,000（6,000）IDU/mg。这一观察结果有力地支持了这两种活动是由同一种物质产生的假设。还已知，由于最纯多肽中含有甲硫氨酸，两者都会通过稀H2 aO 2溶液的作用而失活。并且两者在随后用半胱氨酸盐酸盐还原时完全再活化。
Secretin, cholecystokinin (GCK) and pancreozymin (PZ) are taken up on alginic acid from a dilute acetic acid extract of the heat-coagulated duodeno-jejunal mucosa of the hog. After elution they are precipitated with sodium chloride. From the salt cake secretin is taken up in methyl alcohol. From the material insoluble in methanol witli 5 Ivy dog units (IDU) of CCK per mg, the CCK and PZ activities are adsorbed on CMC cellulose and the active material further chromatographed on TEAE cellulose, resulting in a product with 250 IDU of CCK per mg. Further purification was achieved by passage through a Sephadex G 50 column and subsequent chromatography on an Amberlite XE-64 column. The activity was now 3,000 (6,000) IDU/mg. Since Harper and Raper's original PZ preparation and a commercial sample of Pancreozymin Boots contain 0.3 Ivy dog units of cholecystokinin per mg the cholecystokinin activity has been enriched 10,000-(20,000-)fold. Parallel with this rise in strength of the CCK activity, as assayed in guinea pigs, runs an equal rise in the PZ activity, as determined in cats at three different levels of strength, namely at 0.3. 220 and 3,000 (6,000) IDU/mg. This observation strongly supports the assumption that both activities are exerted by one and the same substance. It is also known that, because of the methionine content of the purest polypeptide, both are inactivated through the action of a dilute H2aO2 solution. and both fully reactivated on subsequent reduction with cysteine hydrochloride.