Tissue plasminogen activator (tPA) attenuates propofol-induced apoptosis in developing hippocampal neurons.
Tissue plasminogen activator (tPA) attenuates propofol-induced apoptosis in developing hippocampal neurons.
复制标题
组织纤溶酶原激活剂 (tPA) 减弱异丙酚诱导的海马神经元凋亡
DOI：
10.1186/s40064-016-2091-y
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Xue Z
中科院分区：
文献类型：
--
作者：
Liang C;Ding M;Du F;Cang J;Xue Z
研究丙泊酚对发育中海马神经元组织型纤溶酶原激活物（tPA）释放的影响，探讨外源性tPA对丙泊酚诱导的海马神经元凋亡的影响。最后，用外源性tPA（5 μg/ml）预处理神经元，然后暴露于丙泊酚（100 μM，6 h）。采用末端转移酶-脱氧尿苷三磷酸-生物素缺口末端标记法（TUNEL）检测海马神经元凋亡，Western blot检测海马神经元裂解型半胱氨酸蛋白酶-3（Cl-Csp 3）蛋白表达，酶联免疫吸附法检测海马神经元培养液中tPA含量。异丙酚降低发育中海马神经元中的tPA水平。结论异丙酚可抑制发育中海马神经元tPA的释放。tPA的加入可部分逆转丙泊酚的凋亡作用。
BackgroundWe investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.MethodsPrimary hippocampal neurons isolated from neonatal Sprague-Dawley rats were exposed to propofol (20, 50, and 100 μM) for 6 h either one time or three times. Finally, neurons were pretreated with exogenous tPA (5 µg/ml), followed by propofol exposure (100 μM, 6 h). The neuron apoptosis was detected by terminal transferase deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and the protein expression of cleaved caspase-3 (Cl-Csp3) was analyzed by western blot, the tPA in media was tested by enzyme-linked immunosorbent assay.ResultsPropofol exposure significantly increased the number of TUNEL-positive neurons and Cl-Csp3 expression in developing hippocampal neurons. Propofol decreased tPA level in the media of developing hippocampal neurons. The neuron appotosis induced by propofol was attenuated by pretreatment of tPA.ConclusionPropofol exposure decreased tPA release in developing hippocampal neurons. The addition of tPA could partially reverse the apoptotic effect of propofol.