A high-throughput 3-D composite dielectrophoretic separator
A high-throughput 3-D composite dielectrophoretic separator
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DOI：
10.1109/tbme.2005.847553
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发表时间：
2005-07-01
影响因子：
4.6
通讯作者：
Hughes, MP
中科院分区：
文献类型：
--
作者：
Fatoyinbo, HO;Kamchis, D;Hughes, MP
介电泳在从生物加工到临床医学的一系列不同领域具有巨大的潜力，但由于产生高度不均匀场所需的微米级电极的低吞吐率而受到阻碍。在这里，我们描述了一种新颖的电极构造方法，使用钻孔层压结构来形成带有电极宽 30 μm、间距 150 μm 的通道。由于这些电极出现在钻孔的所有侧面，因此捕获效率比传统设备有所提高。我们开发并展示了一种分离器，能够以 25 mLhr(-1) 将 50:50 的活酵母细胞和非活酵母细胞混合物分选为 86:14 的混合物。
Dielectrophoresis has great potential to offer a range of diverse fields from bioprocessing to clinical medicine, but is hampered by low throughput rates to the micrometer scale of the electrodes required to generate highly nonuniform fields. Here we describe a novel approach to electrode construction, using a drilled laminated structure to form channels bearing electrodes 30 mu m across and 150 mu m apart. Since these electrodes appear along all sides of the drilled bore, the trapping efficiency is improved over conventional devices. We have developed and demonstrated a separator capable of sorting a 50:50 mixture of viable and nonviable yeast cells into an 86:14 mixture at 25 mLhr(-1).