Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods
Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods
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DOI：
10.1089/1536230041372337
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发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Tsunoda, Y
中科院分区：
文献类型：
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作者：
Kawano, K;Kato, Y;Tsunoda, Y
本研究比较了通过直接注射和细胞融合程序接受胎儿体细胞的核移植猪卵母细胞发育成囊胚的潜力。用德美考辛和蔗糖对体外成熟卵母细胞进行短暂处理后，机械去除含有浓缩染色体团的突起。单个供体细胞在电脉冲后与去核卵母细胞融合，或通过压电驱动显微注射注射到卵母细胞中。直接注射的重建率明显高于细胞融合后的重建率（89% vs. 48%）。然而，核移植卵母细胞发育成囊胚的潜力在注射和融合方法之间没有差异（13% 与 18%）。细胞总数、内细胞团和滋养外胚层。两组间克隆囊胚的细胞数量也没有差异。
The present study compared the potential of nuclear-transferred porcine oocytes receiving fetal somatic cells by direct injection and cell fusion procedures to develop into blastocysts. After brief treatment of in vitro matured oocytes with demecolcine and sucrose, the protrusion containing the condensed chromosome mass was mechanically removed. Single donor cells were fused with enucleated oocytes following electric pulses or injected into oocytes by piezo-actuated microinjection. The reconstruction rate by direct injection was significantly higher than that following cell fusion (89 vs. 48%). The potential of nuclear-transferred oocytes to develop into blastocysts, however, was not different between injection and fusion methods (13% vs. 18%). Total cell number, inner cell mass, and trophectoderm. cell numbers of cloned blastocysts were also not different between the two groups.