Formation of hybrid cell-cell channels.
Formation of hybrid cell-cell channels.
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混合细胞-细胞通道的形成。
DOI：
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发表时间：
1989
影响因子：
11.1
通讯作者：
Gerhard DAHLt
中科院分区：
文献类型：
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作者：
R. Werner;ERic LEVINEt;C. Rabadán;Gerhard DAHLt
卵母细胞细胞-细胞通道测定用于证明连接蛋白-43是细胞-细胞通道形成蛋白，如先前对于连接蛋白-32所示。连接蛋白32在一个和连接蛋白43在另一个卵母细胞的一对的结果在连接电导的形成率相似的观察时，只有一个或另一个连接蛋白在一对的两个卵母细胞表达。这表明在卵母细胞系统中形成了杂交细胞-细胞通道。当连接蛋白-43 mRNA注射的卵母细胞与表达内源性连接蛋白的未注射的卵母细胞配对时，也会形成杂交通道。后者的杂种有明显的两种类型的hemichannels贡献的属性。纯连接蛋白-43通道不是电压门控的，而纯卵母细胞通道是电压依赖性的;这些通道的杂交体整流。
The oocyte cell-cell channel assay was used to demonstrate that connexin-43 is a cell-cell channel-forming protein as previously shown for connexin-32. Expression of connexin-32 in one and connexin-43 in the other oocyte of a pair results in the formation of junctional conductances at rates similar to those observed when only one or the other connexin is expressed in both oocytes of a pair. This suggests that hybrid cell-cell channels form in the oocyte system. Hybrid channels also form when a connexin-43 mRNA-injected oocyte is paired with a noninjected oocyte expressing endogenous connexin. The latter hybrids have properties apparently contributed by both types of hemichannels. Pure connexin-43 channels are not voltage gated, whereas pure oocyte channels are voltage dependent; hybrids of these channels rectify.