Differences of three-dimensional trabecular microstructure in osteopenic rat models caused by ovariectomy and neurectomy
Differences of three-dimensional trabecular microstructure in osteopenic rat models caused by ovariectomy and neurectomy
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DOI：
10.1016/s8756-3282(02)00684-1
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发表时间：
2002-04-01
期刊：
影响因子：
4.1
通讯作者：
Hayashi, K
中科院分区：
文献类型：
--
作者：
Ito, M;Nishida, A;Hayashi, K
我们研究了卵巢切除术（ovx）和坐骨神经切除术（nx）引起的骨质减少病例中骨三维显微结构的差异。将39只8周龄雌性刘易斯大鼠分为两组：（1）去卵巢和假手术组;（2）去卵巢和假手术组。在12周龄时，将这些大鼠处死，以在右颞叶取样。使用微计算机断层扫描的样本，以获得度量参数，如骨体积分数（BV/TV），骨小梁厚度（Tb.Th），和骨小梁分离（Tb.Sp），和非度量参数，如结构模型指数（SMI），骨小梁模式因子（TBPF），和各向异性程度（DA）。所有显微结构参数的变化在ovx组和nx组中均显著，其中BV/TV、Tb.Th、Tb.Sp和SMI的变化在ovx组中比在nx组中更显著，与它们各自的对照相比。Tb.Th在整个分析区域的个体样本的方差系数显著较高，表明小梁变薄发生不均匀，并且由ovx和nx引起的显微结构恶化似乎局部加速，从而诱导小梁穿孔和消失。DA在OVX大鼠中显著增加，而在NX大鼠中降低。两种骨质减少模型的显微结构恶化的外观不同。nx大鼠的三维（3D）图像显示片状骨小梁，而ovx大鼠显示骨小梁弥漫性消失，尤其是在胫骨的中心部分，但保留的骨小梁形状保持不变。nx组皮质面积的减少更为显着。nx和ovx导致骨微观结构的显著变化，显示由于局部加速骨吸收导致的骨小梁穿孔和去除。3D显微计算机断层扫描图像显示了ovx和nx组中发生的不同显微结构变化。骨吸收过程中的负荷增加了各向异性，而制动增加了各向同性。此外，制动对皮质区的影响更显着。(Bone 30：594-598; 2002）（C）2002由Elsevier Science Inc. All rights reserved.
We investigated the differences in three-dimensional microstructure of bone in cases of osteopenia caused by two different procedures: ovariectomy (ovx) and sciatic neurectomy (nx). Thirty-nine 8-week-old female Lewis rats were divided into two groups: (1) ovx and sham operation; and (2) nx and sham operation. At 12 weeks of age these rats were killed to sample the right tibiae. The samples were scanned using microcomputed tomography to obtain metric parameters such as bone volume fraction (BV/TV), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp), and nonmetric parameters such as structure model index (SMI), trabecular bone pattern factor (TBPf), and degree of anisotropy (DA). The changes in all microstructural parameters were significant in both the ovx and nx groups, with those of BV/TV, Tb.Th, Tb.Sp, and SMI more significant in the ovx group than in the nx group, in comparison to their respective controls. The significantly higher coefficient of variance for Tb.Th across the entire analyzed area of the individual samples indicated that the trabecular thinning occurred heterogenously and that the microstructural deterioration induced by ovx and nx appeared to be locally accelerated, so as to induce perforation and disappearance of trabeculae. The DA increased significantly in the ovx rats, whereas it decreased in the nx rats. The appearance of microstructural deterioration differed between the two osteopenic models. The three-dimensional (3D) images from the nx rats showed flake-like trabeculae, whereas the ovx rats exhibited a diffuse disappearance of trabeculae, especially in the central part of the tibia, but with a preservation of shape for those trabeculae that were retained. The reduction in cortical area was more significant in the nx group. nx and ovx resulted in significant changes in bone microstructure, showing perforation and removal of trabeculae due to locally accelerated bone resorption. The 3D microcomputed tomography images demonstrated the different microstructural changes that occurred in the ovx and nx groups. Loading during bone resorption increased the anisotropy, whereas immobilization increased the isotropy. In addition, immobilization had a more significant effect on the cortical area. (Bone 30: 594-598; 2002) (C) 2002 by Elsevier Science Inc. All rights reserved.