Treatment of grape berries, a nonclimacteric fruit with a synthetic auxin, retards ripening and alters the expression of developmentally regulated genes
Treatment of grape berries, a nonclimacteric fruit with a synthetic auxin, retards ripening and alters the expression of developmentally regulated genes
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DOI：
10.1104/pp.115.3.1155
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发表时间：
1997-11-01
期刊：
影响因子：
7.4
通讯作者：
Robinson, SP
中科院分区：
文献类型：
--
作者：
Davies, C;Boss, PK;Robinson, SP
用合成生长素样的化合物苯并噻唑-2-氧乙酸（BTOA）处理葡萄（Vitis Vinifera L.）浆果的处理导致大约2周的成熟发作延迟。这表现为浆果重量，颜色，可变形性和己糖浓度的增加的迟缓。 BTOA治疗也延迟了2周，脱甲酸水平的增加通常伴随成熟并改变了许多发育调控基因的表达。一个通常从浆果套装直至成熟并在成熟后关闭的推定液泡转化酶在BTOA处理的葡萄浆果中一直表达。这种升高的表达导致转化酶活性的水平增加。相比之下，在成熟时，其他四个基因的上调延迟在BTOA处理的水果中延迟。其中包括沙尔酮合酶和UDP-葡萄糖 - 氟葡萄糖3-O-葡萄糖基转移酶，这两者都参与了未知功能的花序氰蛋白合成，几丁质酶和与成熟的与成熟的基因。这些观察结果支持这样一种观点，即生长素（也许与脱落酸结合使用）可能通过影响成熟过程中涉及的基因的表达来控制葡萄浆果成熟。
Treatment of grape (Vitis vinifera L.) berries with the synthetic auxin-like compound benzothiazole-2-oxyacetic acid (BTOA) caused a delay in the onset of ripening of approximately 2 weeks. This was manifested as a retardation of the increases in berry weight, color, deformability, and hexose concentration. BTOA treatment also delayed by 2 weeks the increase in abscisic acid level that normally accompanies ripening and altered the expression of a number of developmentally regulated genes. A putative vacuolar invertase, which is normally expressed from berry set until ripening and turned off after ripening commences, remained expressed throughout development in BTOA-treated grape berries. This elevated expression resulted in increased levels of invertase activity. In contrast, the up-regulation of four other genes normally switched on at the time of ripening was delayed in BTOA-treated fruit. These included chalcone synthase and UDP-glucose-flavonoid 3-O-glucosyl transferase, both of which are involved in anthocyanin synthesis, a chitinase, and a ripening-related gene of an unknown function. These observations support the view that auxins (perhaps in conjunction with abscisic acid) may have a role in the control of grape berry ripening by affecting the expression of genes involved in the ripening process.