SPIKE-AND-WAVE NEOCORTICAL PATTERNS IN RATS - GENETIC AND AMINERGIC CONTROL
SPIKE-AND-WAVE NEOCORTICAL PATTERNS IN RATS - GENETIC AND AMINERGIC CONTROL
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DOI：
10.1016/0306-4522(90)90031-x
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
VADASZ, C
中科院分区：
文献类型：
--
作者：
BUZSAKI, G;LASZLOVSZKY, I;VADASZ, C
在不同年龄的Fischer 344和布法罗品系的近交系大鼠和随机繁殖的Sprague-Dawley品系的大鼠中检查自发发生和药物诱导的高电压棘波脑电图模式。此外，酪氨酸羟化酶活性和多巴胺D2受体密度测定在黑质，纹状体，嗅结节和脑桥区的Fischer 344和布法罗动物。高电压尖峰和波发作存在于87.5%的3个月大和100%的老年Fischer 344大鼠。3个月大的布法罗和Sprague-Dawley动物中完全没有高电压棘波发作，但可通过全身注射戊四氮诱导，并且在年龄较大时，这些菌株的受试者中有58.3%（12个月）和71.4%（> 26个月）出现高电压棘波发作。Fischer 344大鼠的高电压棘波发作的发生率和持续时间显著高于/长于年龄匹配的布法罗和Sprague-Dawley动物。多巴胺受体阻滞剂乙酰丙嗪在3个月大的Fischer 344大鼠中诱导高电压尖峰和波发作的发生率和持续时间增加数倍，但在布法罗大鼠中，当用阈下剂量的戊四氮预处理时，未能诱导高电压尖峰和波发作。酪氨酸羟化酶活性显着高于黑质，纹状体和嗅结节的Fischer 344品系比布法罗大鼠。Fischer 344大鼠的纹状体和嗅结节中酪氨酸羟化酶的活性与D2结合值的显著升高有关。这些研究结果表明，新皮层高电压尖峰和波的表型是遗传介导的和近交系Fischer 344和布法罗大鼠与定义的双线性起源将促进未来的工作，旨在识别参与新皮层高电压尖峰和波事件的产生的遗传因素。显着的基因型.年龄的相互作用支持的建议，然而，高电压尖峰和波事件可能是由一个以上的基因影响，其中一些可能与大脑胺能系统的调节。
Spontaneously occurring and drug-induced high voltage spike-and-wave electroencephalogram patterns were examined in inbred rats of the Fischer 344 and Buffalo strains and of the random-bred Sprague-Dawley strain at different ages. In addition, tyrosine hydroxylase activity and dopamine D2 receptor density were determined in the substantia nigra, corpus striatum, olfactory tubercle and ponsmedulla areas of Fischer 344 and Buffalo animals. High voltage spike-and-wave episodes were present in 87.5% of the 3-month-old and in 100% of the older Fischer 344 rats. High voltage spike-and-wave episodes were completely absent in 3-month-old Buffalo and Sprague-Dawley animals but could be induced by systemic injection of pentylenetetrazol and at an older age they appeared in 58.3% (12-month) and 71.4% (> 26-month) of the subjects of these strains. The incidence and duration of high voltage spike-and-wave episodes were significantly higher/longer in Fischer 344 rats than in the age-matched Buffalo and Sprague-Dawley animals. The dopamine blocker acepromazine induced a several-fold increase of the incidence and duration of high voltage spike-and-wave episodes in 3-month-old Fischer 344 rats, but failed to induce high voltage spike-and-wave episodes in Buffalo rats when they were pretreated with subthreshold doses of pentylenetetrazol. Tyrosine hydroxylase activity was significantly higher in the substantia nigra, corpus striatum and olfactory tubercule of the Fischer 344 strain than in Buffalo rats. The higher tyrosine hydroxylase activity was paralleled with significantly higher D2 binding values in the corpus striatum and olfactory tubercle of Fischer 344 rats. These findings suggest that the neocortical high voltage spike-and-wave phenotype is genetically mediated and that the inbred Fischer 344 and Buffalo rats with defined bilineal origin will facilitate future works aimed at the identification of genetic elements involved in the generation of neocortical high voltage spike-and-wave episodes. The significant genotype .times. age interaction supports the suggestion, however, that high voltage spike-and-wave episodes are likely to be influenced by more than one gene; some of them are probably related to the regulation of brain aminergic systems.