Design and fabrication of devices for investigating cell-sheet stretch
Design and fabrication of devices for investigating cell-sheet stretch
复制标题
研究细胞片拉伸的装置的设计和制造
DOI：
10.1007/s13206-017-1301-1
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发表时间：
2017
期刊：
影响因子：
4.3
通讯作者：
Hideaki Fujita
中科院分区：
文献类型：
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作者：
Yang Liu;Yoshihiro Ojima;Masanobu Horie;Eiji Nagamori;Hideaki Fujita
最近，许多人已经作出了努力，以研究细胞的拉伸反应，通过局灶性粘附，这通常是利用细胞培养在弹性材料。然而，并不是所有的拉伸感应都是通过粘着斑介导的，而是来自细胞-细胞接触，如粘附连接。为了揭示通过细胞接触的拉伸传感机制的细节，我们开发了一种细胞片延伸装置，用于可视化由外部机械拉伸引起的单个活细胞的动态变化。细胞片层是理想的观察对象，因为它可以仅由细胞组成，而不受外部基质的任何机械影响，同时保持正常的细胞-细胞粘附，如在体内情况下。两个微流控延伸装置的设计和制造使用的硅橡胶，这是能够延长由Caco-2细胞制成的细胞片从其原始状态的1.3和1.5倍。基于所获得的结果，我们期望这样的细胞片层延伸系统可以是用于理解通过活细胞中的细胞-细胞接触的细胞拉伸感测机制的有用工具。
Recently, many efforts have been made to investigate the cell stretch response through focal adhesions, which are usually utilized by cells cultured on elastic materials. However, not all stretch sensing is mediated through focal adhesions but from cell-cell contacts such as adherence junctions. To unveil the details of the stretch-sensing mechanism through cellcell contacts, we developed a cell-sheet extension device for visualizing dynamic changes of individual living cells induced by external mechanical stretch. A cell-sheet is an ideal observation object, as it can be comprised merely of cells without any mechanical influences from external matrices while maintaining normal cell-cell adhesions as in thein vivosituation. Two microfluidic extension devices were designed and fabricated using a silicone elastomer, which were capable of extending a cell-sheet made from Caco-2 cells up to 1.3- and 1.5-fold from its original state. Based on the obtained results, we expected that such cell-sheet extension systems can be useful tools for understanding the mechanisms of cellular stretch sensing through cell-cell contacts in living cells.