Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds.
Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds.
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寡核苷酸探针测定鲶鱼池中分离的四环素抗性细菌。
DOI：
10.1006/mcpr.1993.1051
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发表时间：
1993
影响因子：
3.3
通讯作者：
F. Harrell
中科院分区：
文献类型：
--
作者：
A. DePaola;W. Hill;F. Harrell
合成了A、B和C类四环素抗性决定簇的寡核苷酸探针，并单独和组合进行了测试，用于与先前通过限制性片段DNA探针表征的革兰氏阴性分离株杂交。用任一探针类型观察到类似的杂交模式。革兰氏阴性鲶鱼池塘细菌的特点是这些寡核苷酸探针。常见细菌为类志贺邻单胞菌（47.7%）、嗜水气单胞菌（28.8%）和弗氏柠檬酸杆菌（13.3%）。A类（46.4%）四环素耐药决定簇高于B类（19.8%）和C类（0.3%）。每个物种的一些四环素耐药分离株，包括大多数A。大肠杆菌（62.1%）与所有探针均不杂交。
Oligonucleotide probes for class A, B and C tetracycline resistance determinants were synthesized and tested individually and in combination for hybridization with Gram-negative isolates previously characterized by restriction fragment DNA probes. Similar hybridization patterns were observed with either probe type. Gram-negative catfish pond bacteria were characterized by these oligonucleotide probes. The most common bacteria were Plesiomonas shigelloides (47.7%), Aeromonas hydrophila (28.8%) and Citrobacter freundii (13.3%). The class A (46.4%) tetracycline resistance determinant was more prevalent than B (19.8%) and C (0.3%). Some tetracycline-resistant isolates of each species, including most A. hydrophila (62.1%) isolates, failed to hybridize with any of the probes.