Evidence for the presence of only one cysteine residue in the D-type low molecular weight subunits of wheat subunits of wheat glutenin
Evidence for the presence of only one cysteine residue in the D-type low molecular weight subunits of wheat subunits of wheat glutenin
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DOI：
10.1006/jcrs.1998.0224
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发表时间：
1999-01-01
影响因子：
3.8
通讯作者：
Lafiandra, D
中科院分区：
文献类型：
--
作者：
Masci, S;Egorov, TA;Lafiandra, D
面包小麦谷蛋白的d型低分子量亚基具有ω -麦胶蛋白型n端氨基酸序列，但由于存在半胱氨酸残基而被纳入谷蛋白聚合物中。为了确定半胱氨酸残基的数量和位置，从小麦谷蛋白中纯化了id编码的d型低分子量亚基。中国春天使用的程序允许高恢复。MALDI质谱法比较烷基化亚基和未烷基化亚基的分子量表明，每个分子中只存在一个半胱氨酸残基。谷蛋白中D亚基二聚体的检测也支持了这一点。获得了在半胱氨酸之前的62个氨基酸的内部序列，但无法识别半胱氨酸残基，要么是因为它不在所获得的肽的n端测序范围内，要么是因为它存在于两个未识别的位置之一。(C) 1999学术出版社。
D-type low molecular weight subunits of bread wheat glutenin have omega-gliadin type N-terminal amino acid sequences, but are incorporated into the glutenin polymers because of the presence of cysteine residues. In order to determine the number and position of cysteine residues, ID-encoded D-type low molecular weight subunits of wheat glutenin were purified from the cv. Chinese Spring using a procedure thai allowed high recovery. Comparison of the molecular weights of alkylated and unalkylated subunits by MALDI mass spectrometry indicated the presence of only one cysteine residue per molecule. This was supported also by the detection of dimers of D subunits in gluten. An internal sequence of 62 amino acids preceding the cysteine was obtained, but it was nor possible to identify the cysteine residue, either because it was nor within the range of N-terminal sequencing of peptides obtained, or because it was present in one of the two unidentified positions. (C) 1999 Academic Press.