Characterization of a Novel SXT/R391 Integrative and Conjugative Element Carrying cfr, blaCTX-M-65, fosA3, and aac(6′)-Ib-cr in Proteus mirabilis
Characterization of a Novel SXT/R391 Integrative and Conjugative Element Carrying cfr, blaCTX-M-65, fosA3, and aac(6′)-Ib-cr in Proteus mirabilis
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DOI：
10.1128/aac.00849-18
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发表时间：
2018-09-01
影响因子：
4.9
通讯作者：
Wang, Hong-Ning
中科院分区：
文献类型：
--
作者：
Lei, Chang-Wei;Chen, Yan-Peng;Wang, Hong-Ning
在中国猪源奇异变形杆菌中发现了一个新的139，487-bp的SXT/R391整合接合元件ICEPmiChnBCP 11。ICEPmiChnBCP 11携带20种不同的抗菌素耐药基因，包括临床上重要的rRNA甲基转移酶基因cfr、超广谱β-内酰胺酶基因bla（CTX-M-65）、磷霉素耐药基因fosA 3和氟喹诺酮耐药基因aac（6 '）-Ib-cr。将含有各种抗性基因和10个拷贝的IS 26的ISPpu 12介导的复合转座子插入热点4中。ICEPmiChnBCP 11成功地转移到大肠杆菌中。
A novel 139,487-bp SXT/R391 integrative and conjugative element, ICEPmiChnBCP11, was characterized in Proteus mirabilis of swine origin in China. ICEPmiChnBCP11 harbors 20 different antimicrobial resistance genes, including the clinically important rRNA methyltransferase gene cfr, the extended-spectrum beta-lactamase gene bla(CTX-M-65), fosfomycin resistance gene fosA3, and fluoroquinolone resistance gene aac(6')-Ib-cr. An ISPpu12-mediated composite transposon containing various resistance genes and 10 copies of IS26 is inserted in hot spot 4. ICEPmiChnBCP11 was successfully transferred to Escherichia coli.