GENE-TRANSFER INTO THE MEDFLY, CERATITIS-CAPITATA, WITH A DROSOPHILA-HYDEI TRANSPOSABLE ELEMENT
GENE-TRANSFER INTO THE MEDFLY, CERATITIS-CAPITATA, WITH A DROSOPHILA-HYDEI TRANSPOSABLE ELEMENT
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DOI：
10.1126/science.270.5244.2002
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发表时间：
1995-12-22
期刊：
影响因子：
56.9
通讯作者：
SAVAKIS, C
中科院分区：
文献类型：
--
作者：
LOUKERIS, TG;LIVADARAS, I;SAVAKIS, C
利用转座因子Mines的遗传转化系统，将外源功能DNA导入地中海实蝇Ceratitis capitata的胚系染色体中。转化体被鉴定为受体菌株携带的白眼突变的表型回复突变体。在390个筛选用于种系转化的个体的后代中检测到转化体簇。五个独立的和表型活跃的整合事件进行了鉴定，其中每个转座子的单拷贝插入到地中海果蝇基因组的不同位点。分子分析表明，它们代表转座酶介导的转座子插入到地中海果蝇染色体。
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly (medfly) Ceratitis capitata with a germline transformation system based on the transposable element Mines from Drosophila hydei. Transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. Clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. Five independent and phenotypically active integration events were identified, in each of which a single copy of the transposon was inserted into a different site of the medfly genome. Molecular analysis indicates that they represent transposase-mediated insertions of the transposon into medfly chromosomes.