Genome Sequencing of Arabidopsis abp1-5 Reveals Second-Site Mutations That May Affect Phenotypes
Genome Sequencing of Arabidopsis abp1-5 Reveals Second-Site Mutations That May Affect Phenotypes
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DOI：
10.1105/tpc.15.00214
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发表时间：
2015-07-01
期刊：
影响因子：
11.6
通讯作者：
Strader, Lucia C.
中科院分区：
文献类型：
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作者：
Enders, Tara A.;Oh, Sookyung;Strader, Lucia C.
生长素调节植物生长发育的许多方面。多年来，研究生长素结合蛋白1 （ABP1）在生长素应答中的作用一直受到ABP1缺陷突变体胚胎致死性报道的阻碍。然而，在ABP1生长素结合袋（ABP1 -5）中发现了一个有活力的拟南芥TILLING突变体，这使得了解ABP1的功能成为可能。在我们自己对ABP1 -5的研究中，我们观察到生长表型独立于ABP1病变分离，从而使我们对先前描述的ABP1 -5系的基因组进行测序。我们发现ABP1 -5系除了ABP1突变外，还包含超过8000个单核苷酸多态性，其中至少有一些突变可能来自拟南芥Wassilewskija的加入。此外，ABP1 -5背景中的phyB空等位基因可能是先前归因于ABP1功能破坏的长下胚轴表型的原因。我们的研究结果使没有进行补体测试的abp1-5表型的解释复杂化。我们对abp1-5的研究结果也提供了一个警示故事，说明在将作用归因于基因产物时需要使用多个等位基因或互补系。
Auxin regulates numerous aspects of plant growth and development. For many years, investigating roles for AUXIN BINDING PROTEIN1 (ABP1) in auxin response was impeded by the reported embryo lethality of mutants defective in ABP1. However, identification of a viable Arabidopsis thaliana TILLING mutant defective in the ABP1 auxin binding pocket (abp1-5) allowed inroads into understanding ABP1 function. During our own studies with abp1-5, we observed growth phenotypes segregating independently of the ABP1 lesion, leading us to sequence the genome of the abp1-5 line described previously. We found that the abp1-5 line we sequenced contains over 8000 single nucleotide polymorphisms in addition to the ABP1 mutation and that at least some of these mutations may originate from the Arabidopsis Wassilewskija accession. Furthermore, a phyB null allele in the abp1-5 background is likely causative for the long hypocotyl phenotype previously attributed to disrupted ABP1 function. Our findings complicate the interpretation of abp1-5 phenotypes for which no complementation test was conducted. Our findings on abp1-5 also provide a cautionary tale illustrating the need to use multiple alleles or complementation lines when attributing roles to a gene product.