IMMUNOHISTOCHEMICAL ANALYSIS OF MAGNOCELLULAR ELEMENTS IN RAT HYPOTHALAMUS - DISTRIBUTION AND NUMBERS OF CELLS CONTAINING NEUROPHYSIN, OXYTOCIN, AND VASOPRESSIN
IMMUNOHISTOCHEMICAL ANALYSIS OF MAGNOCELLULAR ELEMENTS IN RAT HYPOTHALAMUS - DISTRIBUTION AND NUMBERS OF CELLS CONTAINING NEUROPHYSIN, OXYTOCIN, AND VASOPRESSIN
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DOI：
10.1002/cne.901980106
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发表时间：
1981-01-01
影响因子：
2.5
通讯作者：
PFAFF, DW
中科院分区：
文献类型：
--
作者：
RHODES, CH;MORRELL, JI;PFAFF, DW
用抗催产素（OXY）、抗利尿激素（ADH）和垂体后叶素（neurophysins）的抗血清，用未标记抗体酶技术（PAP）对50只Long-Evans（正常）和Brattleboro（尿崩症）大鼠下丘脑的大细胞成分进行了逐细胞分析。下丘脑的大细胞神经元分布于视上核、室旁核、前连合核和许多副核，并以单个细胞形式分布于下丘脑前区。视神经束将SON分为视交叉后SON和视交叉后SON。在视交叉后的SON中，大多数细胞含有加压素。在SON的主体部分内，催产素产生细胞倾向于在吻侧和背侧被发现，而加压素细胞在尾侧和腹侧更常见。根据细胞形态和肽含量，室旁核可分为内侧、外侧和后三个亚核。室旁核内侧、外侧的大细胞排列紧密，近圆形;室旁核后侧的大细胞排列较分散，呈梭形，长轴走向内外。内侧PVN主要由催产素产生细胞组成，而外侧PVN由加压素产生细胞的核心与催产素细胞的边缘形成。后PVN包含大量催产素产生细胞。ADH和OXY细胞均见于副核。在Long-Evans大鼠中，SON平均有1443个OXY和3236个ADH细胞; PVN有1174个OXY和976个ADH细胞;下丘脑中的辅助大细胞群（包括ACN）有1286个OXY和552个ADH细胞。Brattleboro品系动物在这些细胞核中具有相似数量的细胞。(The在正常动物中含有ADH的细胞在Brattleboro大鼠中被鉴定为大的神经垂体素阴性细胞。大部分大鼠的大细胞催产素和加压素产生细胞位于PVN和SON之外。一个辅助细胞群，特别是ACN，有616个OXY细胞，约为PVN的50%。在每个细胞核中，OXY和ADH细胞的数量之和约为neurophysin细胞的数量。
A cell-by-cell analysis of the magnocellular elements in hypothalami of 50 Long-Evans (normal) and Brattleboro (diabetes insipidis) rats was done using the unlabeled antibody enzyme technique (PAP [peroxidase-antiperoxidase]) with primary antisera directed against oxytocin (OXY), vasopressin (ADH), and the neurophysins. The magnocellular neurons of the hypothalamus were found in the supraoptic (SON), paraventricular (PVN) and anterior comissural (ACN) nuclei, a number of accessory nuclei, and as individual cells in the anterior hypothalamic area. SON was divided by the optic tract into the principal part and retrochiasmatic SON. In retrochiasmatic SON a majority of the cells contained vasopressin. Within the principal part of SON oxytocin-producing cells tended to be found rostrally and dorsally, while the vasopressin cells were more common caudally and ventrally. PVN was divided into 3 subnuclei, the medial, lateral and posterior subnuclei, on the basis of cellular morphology and peptide content. The magnocellular cells of the medial and lateral PVN were closely packed together and nearly round, while those of the posterior PVN were more separated and fusiform in shape with their long axis running in a medio-lateral direction. Medial PVN consisted primarily of oxytocin-producing cells, while lateral PVN was formed by a core of vasopressin-producing cells with a rim of oxytocin cells. Posterior PVN contained largely oxytocin-producing cells. Both ADH and OXY cells were found in the accessory nuclei. In the Long-Evans rat the SON had, on the average, 1443 OXY and 3236 ADH cells; the PVN had 1174 OXY and 976 ADH cells; and the accessory magnocellular groups in the hypothalamus (including the ACN) had 1286 OXY and 552 ADH cells. The Brattleboro strain animal had similar numbers of cells in these nuclei. (The cells which contain ADH in normal animals were identified in the Brattleboro rat as large, neurophysin-negative cells.) A large fraction of the magnocellular oxytocin- and vasopressin-producing cells in the rat were located outside of the PVN and SON. One accessory cell group in particular, ACN, had 616 OXY cells, or about 50% as many as PVN. In each nucleus the sum of the numbers of OXY and ADH cells was approximately the number of neurophysin cells.