Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel.
Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel.
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DOI：
10.1063/1.4891599
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发表时间：
2014-07
期刊：
影响因子：
3.2
通讯作者：
Jian Zhou;P. Giridhar;S. Kasper;I. Papautsky
中科院分区：
文献类型：
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作者：
Jian Zhou;P. Giridhar;S. Kasper;I. Papautsky
在许多生物应用中，细胞过滤是样品制备的关键步骤。在此，我们报告了一个简单的，无过滤器的，基于流体动力惯性迁移的微流控平台。我们的方法建立在两阶段惯性迁移的概念上，它可以精确预测微通道内的微粒位置。我们的设计通过调节低纵横比微通道中旋转引起的升力来操纵较大微粒的平衡位置。在这里，我们证明了微粒的过滤具有极高的效率（>99%）。使用多种前列腺细胞系（LNCaP和人前列腺上皮肿瘤细胞），我们显示了从添加的血液中过滤，浓度为3倍，存活率为>83%。增殖试验的结果显示正常的细胞分裂，并表明对内在特性没有负面影响。考虑到平面低长宽比结构和可预测的聚焦，我们设想有前景的应用，并易于与现有的芯片实验室系统集成。
Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems.