Electrochemical and optical detectors for capillary and chip separations.
Electrochemical and optical detectors for capillary and chip separations.
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DOI：
10.1016/j.trac.2006.11.015
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Xiaomi Xu;Ling Li;S. Weber
中科院分区：
文献类型：
--
作者：
Xiaomi Xu;Ling Li;S. Weber
在毛细管或芯片上的分离中，蛋白质组学领域之外最主要的检测器是电化学（EC）和光学。这些检测器在μM至pM范围内工作，峰值体积为nL，时间分辨率为ms。两类探测器的改进驱动力是不同的。对于EC探测器，该领域试图克服两个限制。一个是电极的始终存在的表面，虽然其催化或吸附特性通常是有利的，但也经常导致灵敏度随时间的变化。另一个是操作电动分离的电气系统与操作检测器的系统的解耦。对于光学探测器，同样存在少量重要的限制。一个是需要将检测系统的便携性（尺寸、重量和功率要求）纳入EC检测器的范围。另一个是拓宽和简化荧光检测的应用，因为它几乎总是涉及衍生化。除了局限性之外，能够使检测器电极和1 μm量级的聚焦激光束以及两种检测器的快速时间响应使毛细管和芯片分离成为小样品、快速、低质量检测限分析的最前沿。
In separations in capillaries or on chips, the most predominant detectors outside of the field of proteomics are electrochemical (EC) and optical. These detectors operate in the μM to pM range on nL peak volumes with ms time resolution. The driving forces for improvement are different for the two classes of detectors. With EC detectors, there are two limitations that the field is trying to overcome. One is the ever-present surface of the electrode which, while often advantageous for its catalytic or adsorptive properties, is also frequently responsible for changes in sensitivity over time. The other is the decoupling of the electrical systems that operate electrokinetic separations from the system operating the detector. With optical detectors, there are similarly a small number of important limitations. One is the need to bring the portability (size, weight and power requirements) of the detection system into the range of EC detectors. The other is broadening and simplifying the applications of fluorescence detection, as it almost always involves derivatization. Limitations aside, the ability to make detector electrodes and focused laser beams of the order of 1 μm in size, and the rapid time response of both detectors have vaulted capillary and chip separations to the forefront of small sample, fast, low mass-detection limit analysis.