Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch.
Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch.
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DOI：
10.1099/mic.0.021790-0
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发表时间：
2009
期刊：
影响因子：
1.5
通讯作者：
John A. Wright;A. Grant;D. Hurd;M. Harrison;Edward Guccione;D. Kelly;D. Maskell
中科院分区：
文献类型：
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作者：
John A. Wright;A. Grant;D. Hurd;M. Harrison;Edward Guccione;D. Kelly;D. Maskell
空肠弯曲菌是人类食源性腹泻的常见原因。对生物体的生理和代谢能力的了解是有限的。我们报告了对分批培养中空肠弯曲菌生长周期的详细分析。转录组学、表型和代谢分析相结合，证明了一个高度动态的“静止期”，其特征是运动峰值、大量基因表达变化和底物转换，尽管转录物变化表明静止期开始时代谢下调。细菌运动的视频跟踪可识别静止期的峰值活动。培养物上清液的氨基酸分析显示氨基酸利用的优先顺序。质子核磁共振 (1H-NMR) 强调了乙酸盐转换机制，细菌从乙酸盐排泄转变为乙酸盐摄取，很可能是对其他底物耗尽的反应。乙酸盐的生产需要 pta (Cj0688) 和 ackA (Cj0689)，但不需要 acs 同源物 (Cj1537c)。与野生型空肠弯曲菌相比，Cj0688 和 Cj0689 中的插入突变体在生长周期的稳定期和衰退期维持活力的能力较差，这表明这些基因和乙酸盐途径对于生存很重要。
Campylobacter jejuni is a prevalent cause of food-borne diarrhoeal illness in humans. Understanding of the physiological and metabolic capabilities of the organism is limited. We report a detailed analysis of the C. jejuni growth cycle in batch culture. Combined transcriptomic, phenotypic and metabolic analysis demonstrates a highly dynamic 'stationary phase', characterized by a peak in motility, numerous gene expression changes and substrate switching, despite transcript changes that indicate a metabolic downshift upon the onset of stationary phase. Video tracking of bacterial motility identifies peak activity during stationary phase. Amino acid analysis of culture supernatants shows a preferential order of amino acid utilization. Proton NMR (1H-NMR) highlights an acetate switch mechanism whereby bacteria change from acetate excretion to acetate uptake, most probably in response to depletion of other substrates. Acetate production requires pta (Cj0688) and ackA (Cj0689), although the acs homologue (Cj1537c) is not required. Insertion mutants in Cj0688 and Cj0689 maintain viability less well during the stationary and decline phases of the growth cycle than wild-type C. jejuni, suggesting that these genes, and the acetate pathway, are important for survival.