Effects of dietary arginine supplementation on growth performance, flesh quality, muscle antioxidant capacity and antioxidant-related signalling molecule expression in young grass carp (Ctenopharyngodon idella)
Effects of dietary arginine supplementation on growth performance, flesh quality, muscle antioxidant capacity and antioxidant-related signalling molecule expression in young grass carp (Ctenopharyngodon idella)
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DOI：
10.1016/j.foodchem.2014.06.091
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发表时间：
2015-01-15
期刊：
影响因子：
8.8
通讯作者：
Zhou, Xiao-Qiu
中科院分区：
文献类型：
--
作者：
Wang, Biao;Liu, Yang;Zhou, Xiao-Qiu
以草鱼幼鱼为试验对象，研究了不同水平精氨酸（6.9- 24.5g/kg）对草鱼生长性能、肌肉品质、抗氧化状态和抗氧化相关信号分子表达的影响。适量精氨酸可显著提高肌肉蛋白质、脂肪和一氧化氮含量、剪切力、羟脯氨酸浓度和pH值。蒸煮损失、乳酸含量、组织蛋白酶活性、丙二醛和蛋白质羰基含量则呈现相反的变化趋势。适宜精氨酸水平显著提高了肌肉中谷胱甘肽含量、铜/锌超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性和基因表达。此外，适量精氨酸可显著提高肌肉中谷氨酸-半胱氨酸连接酶、雷帕霉素靶蛋白、核糖体蛋白S6激酶1、酪蛋白激酶2和NF-E2相关因子2的表达水平。然而，最佳精氨酸显着降低肌肉中Kelch样ECH相关蛋白1 mRNA水平。总之，精氨酸改善了肉品质和肌肉抗氧化能力，并调节抗氧化相关信号分子的表达。(C)2014爱思唯尔有限公司版权所有。
Growth performance, flesh quality, antioxidant status and antioxidant-related signalling molecule expression in the muscle of young grass carp, which were fed graded levels of arginine (6.9-24.5 g/kg diet) for eight weeks, were investigated. Muscle protein, lipid and nitric oxide contents, shear force, hydroxyproline concentration, and pH were significantly improved by appropriate arginine. Cooking loss, lactate content, cathepsins activities, malondialdehyde and protein carbonyl contents exhibited an opposite tendency. Additionally, optimum arginine significantly enhanced glutathione content and the activities and gene expression of copper/zinc superoxide dismutase, catalase and glutathione peroxidase in muscle. Moreover, the expression levels of glutamate-cysteine ligase, target of rapamycin, ribosome protein S6 kinase 1, casein kinase 2 and NF-E2-related factor 2 in muscle were significantly elevated by appropriate arginine. However, optimum arginine significantly decreased Kelch-like ECH-associated protein 1 mRNA levels in muscle. In conclusion, arginine improved the flesh quality and muscle antioxidant capacity and regulated antioxidant-related signalling molecule expression. (C) 2014 Elsevier Ltd. All rights reserved.