The effect of herpesvirus infection on the expression of cell adhesion molecules on cultured human dermal microvascular endothelial cells
The effect of herpesvirus infection on the expression of cell adhesion molecules on cultured human dermal microvascular endothelial cells
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DOI：
10.1016/s0923-1811(00)00080-3
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发表时间：
2000-09-01
影响因子：
4.6
通讯作者：
Lee, KH
中科院分区：
文献类型：
--
作者：
Kim, YC;Bang, D;Lee, KH
细胞介导的对单纯疱疹病毒（HSV）的免疫应答可能在疱疹性角膜炎、多形性红斑或白塞氏病的发病机制中起重要作用。我们研究了疱疹病毒感染是否调节细胞粘附分子在培养的人真皮微血管内皮细胞（HDMEC）的表达和调节T淋巴细胞与HDMEC结合。细胞间粘附分子1（ICAM-1）、血管细胞粘附分子1（VCAM-1）的表达。HSV-1、HSV-2或麻疹病毒处理后HDMEC上的E-selectin和E-selectin显著升高。抗IL-1 α抗体或抗TNF α抗体部分抑制HDMEC上ICAM-1、VCAM-1或E-选择素的表达。HDMEC经HSV-1或麻疹病毒处理后，T淋巴细胞与HDMEC的结合明显增强。在用针对HDMEC的抗ICAM-1抗体、抗IL-1 α抗体或抗TNF α抗体处理后孵育16小时后，T淋巴细胞与HDMEC的结合被显著抑制。这些结果提示，HSV可诱导HDMEC表面ICAM-1的表达增加，或诱导VCAM-1和E-选择素的表达，其中ICAM-1的表达主要调节T淋巴细胞与HDMEC的结合。HSV感染HDMEC后产生的IL-1 α或TNF α可能与这些事件有关。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Cell-mediated immune response to herpes simplex virus (HSV) may be important in the pathogenesis of herpes keratitis, erythema multiforme or Behcet's disease. We examined whether herpesvirus infection regulates the expression of cell adhesion molecules on cultured human dermal microvascular endothelial cells (HDMEC) and the regulation of T-lymphocytes binding to HDMEC. The expression of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1). or E-selectin on HDMEC increased significantly after treatment with HSV-1, HSV-2, or measles virus on HDMEC. Anti-IL-1 alpha antibody or anti-TNF alpha antibody partially inhibited the expression of ICAM-1, VCAM-1, or E-selectin on HDMEC. The binding of T-lymphocytes to HDMEC increased significantly after the treatment of HSV-1 or measles virus on HDMEC. The binding of T-lymphocytes to HDMEC was significantly inhibited after 16 h of incubation following treatment with anti-ICAM-1 antibody, anti-IL-1 alpha antibody or anti-TNF alpha antibody to HDMEC. These study results suggest that HSV induces the increased expression of ICAM-1, or induction of VCAM-1 and E-selectin on HDMEC and that among these adhesion molecules, the expression of ICAM-1 on HDMEC mainly regulates the binding of T-lymphocytes to HDMEC, The data also suggest that IL-1 alpha or TNF alpha which was produced by HSV infected HDMEC may be related to these events. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.