Interleukin-11, IL-11 receptorα and leukemia inhibitory factor are dysregulated in endometrium of infertile women with endometriosis during the implantation window
Interleukin-11, IL-11 receptorα and leukemia inhibitory factor are dysregulated in endometrium of infertile women with endometriosis during the implantation window
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DOI：
10.1016/j.jri.2005.07.004
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Salamonsen, LA
中科院分区：
文献类型：
--
作者：
Dimitriadis, E;Stoikos, C;Salamonsen, LA
白细胞介素 (IL)-11 对于小鼠胚胎植入至关重要，有证据表明它在人类胚胎植入中也发挥着重要作用。本研究评估了患有子宫内膜异位症 (I/E) 的不孕女性和正常生育女性（对照）在着床窗口期子宫内膜中免疫反应性 IL-11、IL-11 受体 (R) a 和白血病抑制因子 (LIF) 的情况。 I/E (N = 7) 的子宫内膜活检从 LH 激增开始计时，并在排卵后 (POD) 5-10 天进行。对照活检 (N = 8) 是在月经周期的第 19 天至 24 天之间进行的。使用半定量免疫组织化学评分评估 IL-11、IL-11Ra 和 LIF 的染色强度。免疫反应性 IL-11、IL-11R α 和 LIF 主要存在于腺上皮中，而管腔上皮则呈现斑片状染色。所有对照的腺上皮细胞中 IL-11、IL-11R α 和 LIF 均呈阳性染色。 I/E 组中腺上皮不存在 IL-11 和 IL-11R α 染色。与对照组相比，I/E 中腺上皮的 LIF 染色强度显着较低。
Interleukin (IL)-11 is essential for embryo implantation in the mouse and evidence suggests it has a role in implantation in humans. This study has evaluated immunoreactive IL-11, IL-11 receptor (R) a and leukemia inhibitory factor (LIF) in endometrium of infertile women with endometriosis (I/E) and normal fertile women (controls) during the implantation window. Endometrial biopsies from I/E (N = 7) were timed from the LH surge and were post-ovulatory days (POD) 5-10. Control biopsies (N = 8) from women were between days 19 and 24 of the menstrual cycle. Staining intensity of IL-11, IL-11Ra and LIF evaluated using semi-quantitative immunohistochemistry scores. Immunoreactive IL-11, IL-11R alpha and LIF were present predominantly in glandular epithelium, while luminal epithelium showed patchy staining. All controls stained positively for IL-11, IL-11R alpha and LIF in glandular epithelium. IL-11 and IL-11R alpha staining was absent from glandular epithelium in cohorts of I/E. LIF staining intensity in glandular epithelium was significantly lower in I/E compared to controls.