New class of Bacillus subtilis glutamine-requiring mutants
New class of Bacillus subtilis glutamine-requiring mutants
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新一类枯草芽孢杆菌需要谷氨酰胺突变体
DOI：
10.1128/jb.148.2.653-658.1981
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发表时间：
1981
影响因子：
3.2
通讯作者：
G. Reysset
中科院分区：
文献类型：
--
作者：
G. Reysset
通过遗传分析，从枯草芽孢杆菌168分离的8个谷氨酰胺需要突变体的突变都显示与thyA标记连锁。用其中一个谷氨酰胺突变进行的三因素转导分析表明，B.枯草芽孢杆菌染色体为gltA-thyA-gln。重组指数值的基础上，两个紧密连锁的群体进行了鉴定。属于一组的突变被分配到谷氨酰胺合成酶的结构基因，而属于另一组的突变可能会损害一个调控位点。两个重组组突变体的谷氨酰胺合成酶残基活性和交叉反应物质支持了上述结论。
By using genetic analysis, the mutations of eight glutamine-requiring mutants isolated from Bacillus subtilis 168 were all shown to be linked to the thyA marker. A three-factor transduction analysis performed with one of the gln mutations indicated that the gene order in this region of the B. subtilis chromosome was gltA-thyA-gln. On the basis of recombination index values, two closely linked groups were identified. The mutations belonging to one group were assigned to the structural gene for glutamine synthetase, and those belonging to the other group might impair a regulatory locus. The residual glutamine synthetase activities and the cross-reacting materials of the mutants from both recombination groups supported these conclusions.