Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue
Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue
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DOI：
10.1186/s12958-019-0487-6
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发表时间：
2019-06-12
影响因子：
4.4
通讯作者：
Duncan, Francesca E.
中科院分区：
文献类型：
--
作者：
Dipali, Shweta S.;Ferreira, Christina R.;Duncan, Francesca E.
生殖衰老是一种发生在所有女性身上的强健表型，其特征是配子数量和质量显著降低，这会对内分泌功能和生育能力产生负面影响。卵母细胞、卵泡和卵巢中与年龄相关的差异已经被很好地记录下来，但更广泛的环境如何随着年龄的变化而变化的了解较少。脂肪是体内最大的器官之一，性腺周围的脂肪组织包围着啮齿动物的卵巢，构成了一个局部的卵巢环境。本研究的目的是研究卵巢周围脂肪组织如何随着生殖年龄的增加而发生变化。方法我们分离了两组CB6F1小鼠的性腺周围脂肪组织：生殖幼鼠(6-12周)和生殖大鼠(14-17个月)。进行比较组织学分析以评估脂肪细胞的结构。然后我们从组织中提取脂肪，并进行多反应监测(MRM)分析，这是一种基于质谱学的代谢物分析方法，以比较这些年龄组性腺周围脂肪组织的脂肪分布情况。结果我们发现，高龄生殖年龄与脂肪细胞肥大和单位面积脂肪细胞数量的相应减少有关。在所检测的10种脂类中，三酰甘油(TAG)在青年和老年人群中的分布有显著差异，尽管定量分析显示每重量性腺周围脂肪组织的TAG总量随着年龄的增加而减少。结论这些发现准确地揭示了与年龄相关的性腺周围脂肪组织在脂肪细胞形态和脂类分布方面的生理变化，为进一步研究这些变化如何影响脂肪细胞和卵巢功能奠定了基础。
BackgroundReproductive aging is a robust phenotype that occurs in all females and is characterized by a significant reduction in gamete quantity and quality, which can have negative consequences on both endocrine function and fertility. Age-associated differences in the oocyte, follicle, and ovary have been well-documented, but how the broader environment changes with age is less well understood. Fat is one of the largest organs in the body, and peri-gonadal adipose tissue surrounds the rodent ovary and comprises a local ovarian environment. The goal of this study was to characterize how peri-ovarian adipose tissue changes with advanced reproductive age.MethodsWe isolated peri-gonadal adipose tissue from two cohorts of CB6F1 mice: reproductively young (6-12weeks) and reproductively old (14-17months). A comparative histological analysis was performed to evaluate adipocyte architecture. We then extracted lipids from the tissue and performed multiple reaction monitoring (MRM)-profiling, a mass spectrometry-based method of metabolite profiling, to compare the lipid profiles of peri-gonadal adipose tissue in these age cohorts.ResultsWe found that advanced reproductive age was associated with adipocyte hypertrophy and a corresponding decrease in the number of adipocytes per area. Of the 10 lipid classes examined, triacylglycerols (TAGs) had significantly different profiles between young and old cohorts, despite quantitative analysis revealing a decrease in the total amount of TAGs per weight of peri-gonadal adipose tissue with age.ConclusionsThese findings pinpoint age-associated physiological changes in peri-gonadal adipose tissue with respect to adipocyte morphology and lipid profiles and lay the foundation for future studies to examine how these alterations may influence both adipocyte and ovarian function.