Metabolic adaptation of Mycobacterium avium subsp. paratuberculosis to the gut environment.
Metabolic adaptation of Mycobacterium avium subsp. paratuberculosis to the gut environment.
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鸟分枝杆菌副结核亚种对肠道环境的代谢适应
DOI：
10.1099/mic.0.062737-0
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发表时间：
2013
期刊：
影响因子：
1.5
通讯作者：
Goethe R
中科院分区：
文献类型：
--
作者：
Weigoldt M;Meens J;Bange FC;Pich A;Gerlach GF;Goethe R
关于致病性分枝杆菌对自然宿主环境适应性的蛋白质组学水平的知识是有限的。aviumsubsp分枝杆菌。副结核（MAP）引起约翰氏病，一种慢性和无法治愈的反刍动物肉芽肿性肠炎，并被认为是人类克罗恩病的推定病原。采用LC-MS-MS和2D差异凝胶电泳（DIGE）技术，对病牛胃肠道制备的MAP临床菌株的蛋白谱与体外培养的相同菌株的蛋白谱进行了比较。LC-MS-MS分析表明，两种条件下均存在中心碳代谢途径的主要酶，包括糖酵解、糖异生、三羧酸循环和戊糖磷酸途径。此外，在体内和体外检测到广泛的脂质β-氧化酶，其中9种已被证明是分枝杆菌在胆固醇上生长所必需的。使用2D-DIGE，我们发现几种关键酶的水平增加，表明MAP对宿主的适应。其中，FadE5、FadE25和AdhB表明胆固醇在牛肠粘膜中作为碳源；呼吸酶AtpA、NuoG和SdhA提示感染期间呼吸增加。此外，戊糖磷酸途径酶Gnd2、Zwf和Tal以及KatG、SodA和GroEL水平的升高表明MAPin在体内具有强烈的应激反应。总之，我们的研究结果为致病性分枝杆菌在其天然宿主中的代谢适应提供了新的见解。
Knowledge on the proteome level about the adaptation of pathogenic mycobacteria to the environment in their natural hosts is limited.Mycobacterium aviumsubsp.paratuberculosis(MAP) causes Johne’s disease, a chronic and incurable granulomatous enteritis of ruminants, and has been suggested to be a putative aetiological agent of Crohn’s disease in humans. Using a comprehensive LC-MS-MS and 2D difference gel electrophoresis (DIGE) approach, we compared the protein profiles of clinical strains of MAP prepared from the gastrointestinal tract of diseased cows with the protein profiles of the same strains after they were grownin vitro. LC-MS-MS analyses revealed that the principal enzymes for the central carbon metabolic pathways, including glycolysis, gluconeogenesis, the tricaboxylic acid cycle and the pentose phosphate pathway, were present under both conditions. Moreover, a broad spectrum of enzymes for β-oxidation of lipids, nine of which have been shown to be necessary for mycobacterial growth on cholesterol, were detectedin vivoandin vitro. Using 2D-DIGE we found increased levels of several key enzymes that indicated adaptation of MAP to the host. Among these, FadE5, FadE25 and AdhB indicated that cholesterol is used as a carbon source in the bovine intestinal mucosa; the respiratory enzymes AtpA, NuoG and SdhA suggested increased respiration during infection. Furthermore higher levels of the pentose phosphate pathway enzymes Gnd2, Zwf and Tal as well as of KatG, SodA and GroEL indicated a vigorous stress response of MAPin vivo. In conclusion, our results provide novel insights into the metabolic adaptation of a pathogenic mycobacterium in its natural host.
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影响因子：
14
作者：
Boeer, Ulrike;Lohrenz, Andrea;Wilhelmi, Mathias
通讯作者：
Wilhelmi, Mathias
分枝杆菌中的过氧化还原蛋白系统。
影响因子：
--
作者：
T. Jaeger
通讯作者：
T. Jaeger
影响因子：
2.2
作者：
Granger, K;Moore, RJ;Tizard, MLV
通讯作者：
Tizard, MLV
影响因子：
2.4
作者：
BUERGELT, CD;HALL, C;DUNCAN, JR
通讯作者：
DUNCAN, JR
影响因子：
30.3
作者：
Russell DG;VanderVen BC;Lee W;Abramovitch RB;Kim MJ;Homolka S;Niemann S;Rohde KH
通讯作者：
Rohde KH