Type 2C phosphatase 1 of Artemisia annua L. is a negative regulator of ABA signaling.
Type 2C phosphatase 1 of Artemisia annua L. is a negative regulator of ABA signaling.
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DOI：
10.1155/2014/521794
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发表时间：
2014
影响因子：
--
通讯作者：
Tang K
中科院分区：
文献类型：
--
作者：
Zhang F;Fu X;Lv Z;Shen Q;Yan T;Jiang W;Wang G;Sun X;Tang K
植物激素脱落酸（ABA）在植物发育和环境胁迫反应中发挥着重要作用。此外，ABA 还调节药用植物青蒿中的次生代谢，例如青蒿素。虽然早期的研究表明 ABA 受体 AaPYL9 在 ABA 诱导的青蒿素含量提高中发挥积极作用，但青蒿中 ABA 信号通路中的许多成分仍有待阐明。为了深入了解 AaPYL9 的功能，我们分离并表征了 AaPYL9 的相互作用伙伴， AaPP2C1。 AaPP2C1的编码序列编码464个氨基酸的推导蛋白，具有植物类型进化枝A PP2C的所有特征。转录分析表明，ABA、盐和干旱处理后 AaPP2C1 的表达水平增加。酵母双杂交和双分子荧光互补测定 (BiFC) 显示 AaPYL9 与 AaPP2C1 相互作用。 AaPYL9 中的 P89S、H116A 取代以及 AaPP2C1 中第三个磷酸化位点样基序的 G199D 取代或删除消除了这种相互作用。此外，与野生型相比，AaPP2C1 的组成型表达赋予 ABA 不敏感性。总之，我们的数据表明，AaPP2C1 是 AaPYL9 相互作用的伙伴，参与青蒿中 ABA 信号通路的负调节。
The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. Additionally, ABA also regulates secondary metabolism such as artemisinin in the medicinal plant Artemisia annua L. Although an earlier study showed that ABA receptor, AaPYL9, plays a positive role in ABA-induced artemisinin content improvement, many components in the ABA signaling pathway remain to be elucidated in Artemisia annua L. To get insight of the function of AaPYL9, we isolated and characterized an AaPYL9-interacting partner, AaPP2C1. The coding sequence of AaPP2C1 encodes a deduced protein of 464 amino acids, with all the features of plant type clade A PP2C. Transcriptional analysis showed that the expression level of AaPP2C1 is increased after ABA, salt, and drought treatments. Yeast two-hybrid and bimolecular fluorescence complementation assays (BiFC) showed that AaPYL9 interacted with AaPP2C1. The P89S, H116A substitution in AaPYL9 as well as G199D substitution or deletion of the third phosphorylation site-like motif in AaPP2C1 abolished this interaction. Furthermore, constitutive expression of AaPP2C1 conferred ABA insensitivity compared with the wild type. In summary, our data reveals that AaPP2C1 is an AaPYL9-interacting partner and involved in the negative modulation of the ABA signaling pathway in A. annua L.