Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative Patients.
Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative Patients.
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评估导致食物过敏性的机制的蛋白质组学方法：放射性过敏吸附剂测试阳性和阴性患者的 2D-DIGE 比较分析。
DOI：
10.1155/2011/673618
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Schwartz,StanleyA
中科院分区：
文献类型：
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作者：
Nair,Bindukumar;Wheeler,JohnC;Sykes,DonaldE;Brown,Paula;Reynolds,JessicaL;Aalinkeel,Ravikumar;Mahajan,SupriyaD;Schwartz,StanleyA
使用2D-DIGE分析比较具有严重食物过敏的RAST+受试者和RAST−受试者的蛋白质组谱，以获得食物过敏特异性的候选生物标志物。 我们的分析突出了RAST+和RAST−组之间差异表达的52种蛋白质，其中37种被成功鉴定，包括硫酸软骨素、锌指蛋白、C型凝集素、视黄酸结合蛋白、热休克蛋白、肌球蛋白、细胞因子、肥大细胞表达蛋白和MAP激酶。生物网络分析工具Metacore 揭示了大多数这些受调节的蛋白质在免疫耐受、超敏反应中发挥作用，并调节诱导Th 2应答的细胞因子模式，Th 2应答通常导致IgE介导的过敏反应，其与食物过敏有直接或间接的生物学联系。通过生物信息学分析确定与某些过敏表型和潜在交叉反应蛋白相关的独特生物标志物将为食物过敏患者的过敏反应机制提供巨大的洞察力。
Proteomic profiles of RAST+subjects with severe food allergies and RAST−subjects were compared using 2D‐DIGE analysis to obtain candidate biomarkers specific to food allergies. Our analysis highlighted 52 proteins that were differentially expressed between the RAST+and RAST−groups of which 37 were successfully identified that include chondroitin sulfates, zinc finger proteins, C‐type lectins, retinoic acid binding proteins, heat shock proteins, myosin, cytokines, mast cell expressed proteins, and MAP kinases. Biological network analysis tool Metacore revealed that most of these regulated proteins play a role in immune tolerance, hypersensitivity and modulate cytokine patterns inducing a Th2 response that typically results in IgE‐mediated allergic response which has a direct or indirect biological link to food allergy. Identifying unique biomarkers associated with certain allergic phenotypes and potentially cross‐reactive proteins through bioinformatics analyses will provide enormous insight into the mechanisms that underlie allergic response in patients with food allergies.