Simultaneous molecular MRI of extracellular matrix collagen and inflammatory activity to predict abdominal aortic aneurysm rupture
Simultaneous molecular MRI of extracellular matrix collagen and inflammatory activity to predict abdominal aortic aneurysm rupture
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DOI：
10.1038/s41598-020-71817-x
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发表时间：
2020-09-16
影响因子：
4.6
通讯作者：
Makowski, Marcus R.
中科院分区：
文献类型：
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作者：
Adams, Lisa C.;Brangsch, Julia;Makowski, Marcus R.
腹主动脉瘤（AAA）是一种危及生命的血管疾病，在破裂的情况下死亡率高达80%。目前的生物标志物不能解释与尺寸无关的破裂风险。通过结合不同分子探针的信息，多靶点分子MRI具有使AAA个体化表征成为可能的潜力。在这项实验研究中，我们的目的是检查细胞外胶原蛋白和炎症的同时成像的可行性，用于预测小鼠AAA破裂风险的大小无关性。研究设计包括：（1）一项基于结果的纵向研究，一周后进行一次成像，随访和死亡作为评估破裂风险的终点。(2)在1、2、3和4周后通过成像表征AAA发展的逐周研究。对于这两项研究，在一次成像中，对动物施用1型胶原靶向钆基探针（细胞外基质（ECM）重塑的替代标记物）和氧化铁基探针（炎症活性的替代标记物）。胶原和氧化铁探针的体内测量结果显示与离体组织学显著相关（p
Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with an up to 80% mortality in case of rupture. Current biomarkers fail to account for size-independent risk of rupture. By combining the information of different molecular probes, multi-target molecular MRI holds the potential to enable individual characterization of AAA. In this experimental study, we aimed to examine the feasibility of simultaneous imaging of extracellular collagen and inflammation for size-independent prediction of risk of rupture in murine AAA. The study design consisted of: (1) A outcome-based longitudinal study with imaging performed once after one week with follow-up and death as the end-point for assessment of rupture risk. (2) A week-by-week study for the characterization of AAA development with imaging after 1, 2, 3 and 4 weeks. For both studies, the animals were administered a type 1 collagen-targeted gadolinium-based probe (surrogate marker for extracellular matrix (ECM) remodeling) and an iron oxide-based probe (surrogate marker for inflammatory activity), in one imaging session. In vivo measurements of collagen and iron oxide probes showed a significant correlation with ex vivo histology (p