COMBINATION OF C-PARVUM AND SPECIFIC IMMUNIZATION AGAINST ARTIFICIAL PULMONARY METASTASES IN MICE
COMBINATION OF C-PARVUM AND SPECIFIC IMMUNIZATION AGAINST ARTIFICIAL PULMONARY METASTASES IN MICE
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DOI：
10.1002/ijc.2910160506
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发表时间：
1975-01-01
影响因子：
6.4
通讯作者：
WITHERS, HR
中科院分区：
文献类型：
--
作者：
MILAS, L;KOGELNIK, HD;WITHERS, HR
我们研究了腹腔内进行特异性免疫是否可以增强C.的活性。 parvum（0.25mg腹膜内或静脉内）针对C3Hf/Bu小鼠中静脉注射的同基因纤维肉瘤细胞，表现为肺转移（结节、集落）的减少和/或受体存活时间的延长。在活肿瘤细胞 IV 接种之前或之后应用特异性免疫和微小隐孢子虫的组合比单一治疗更有效。与单独注射活细胞相比，静脉注射重度辐射和活肿瘤细胞的混合物在正常小鼠的肺部产生更多的肿瘤结节。在之前用微小念珠菌治疗的小鼠中没有发现混合照射细胞的转移增强作用，如果在肿瘤细胞后注射免疫刺激剂，这种作用就会被消除。在腿部已经患有这种肿瘤的小鼠中，通过静脉注射纤维肉瘤细胞而产生的肺转移瘤减少了。通过用微小念珠菌治疗受体，可以增加这种对转移的伴随免疫力，但用辐照细胞处理却不会增加：而且，将辐照细胞与微小念珠菌一起注射并不能提高后者的效率。 C. parvum 在 T 细胞剥夺中不如对照小鼠有效，这表明在该系统中，T 细胞是这种免疫刺激剂发挥最佳抗肿瘤活性所必需的。特异性免疫对 T 细胞剥夺的小鼠无效，也不能提高小隐孢子虫的效率。
We have studied whether specific immunization administered intraperitoneally can augment the activity ofC. parvum (0.25 mg intraperitoneally or intravenously) against intravenously injected cells of a syngeneic fibrosarcoma in C3Hf/Bu mice as expressed by the reduction of pulmonary metastases (nodules, colonies) and/or by the prolongation of the survival of recipients. Combination of specific immunization and C. parvum, applied either before or after IV inoculation of viable tumor cells, was more effective than the single treatments. IV injection of a mixture of heavily irradiated and viable tumor cells gave more tumor nodules in the lungs of normal mice than injection of viable cells alone. The metastasis‐enhancing effect of admixed irradiated cells was not found in mice previously treated with C. parvum, and was abolished if the immunostimulant was injected after tumor cells. Generation of lung metastases by IV inoculation of fibrosarcoma cells was reduced in mice already having this tumor in the leg. This concomitant immunity to metastases was increased by treating the recipients with C. parvum, but not with irradiated cells: also, the injection of irradiated cells together with C. parvum did not augment the efficiency of the latter. C. parvum was not as effective in T‐cell deprived as in control mice, which suggests that in this system, T‐cells are required for optimal anti‐tumor activity of this immunostimulant. Specific immunization was not effective in T‐cell‐deprived mice and did not augment the efficiency of C. parvum.