Comparative genetic diversity of Triticum aestivum-Triticum polonicum introgression lines with long glume and Triticum petropavlovskyi by AFLP-based assessment
Comparative genetic diversity of Triticum aestivum-Triticum polonicum introgression lines with long glume and Triticum petropavlovskyi by AFLP-based assessment
复制标题
DOI：
10.1007/s10722-007-9221-x
复制
发表时间：
2008-02-01
影响因子：
2
通讯作者：
Furuta, Y.
中科院分区：
文献类型：
--
作者：
Akond, A. S. M. G. Masum;Watanabe, N.;Furuta, Y.
小麦（Triticum aestivum L./T）一组渗入系的遗传多样性。具有长颖片的蓼属植物和petropavlovskyi。et Migusch。经扩增片段长度多态性（AFLP）分析。在F-6代以前，一直采用小规模的群体育种方法培育渐渗系。采用4种引物组合对38株长颖片型六面体F-7植物及其亲本进行了AFLP分析。亲本间共检测到47个多态性位点，其中15个位点在38个品系间发生渐渗。据推测，大约50%的A或B基因组相关多态性位点是渐渗的。渐渗系的变异仅限于亲本T. aestivum L. cv之间的多样性。Novosibirskaya 67 （N67）和T. polonicum L. cv。IC12196。N67比IC12196更接近38个渐渗系。UPGMA聚类和主坐标分析（PCO）分组结果表明，N67与遗传基因的相似性值为0.84 ~ 0.98。11份T. petropavlovskyi资料与UPGMA聚类和PCO聚类的渐近系相区分，T. petropavlovskyi位于渐近系与IC12196之间。一些渐渗线在遮阳篷和颖片长度上与T. petropavlovskyi相似。38个渐渗系间的遗传变异范围明显大于T. petropavlovskyi。我们认为，petropavlovskyi是由稻瘟霉与稻瘟霉杂交的精耕细作而建立的。在波兰。
Genetic diversity of a set of introgression lines of Triticum aestivum L./T. polonicum L. with long glume and T. petropavlovskyi Udacz. et Migusch. were analyzed by Amplified Fragments Length Polymorphism (AFLP). Small-scale bulk breeding method was applied throughout until F-6 generation to develop the introgression lines. Thirty-eight hexapolid F-7 plants with long glume phenotype and their parents were subjected to AFLP analysis by four primer combinations. A total of 47 polymorphic loci were detected between the parents, 15 of them were introgressed across the 38 lines. It was hypothesized that approximately 50% of A or B genomes associated polymorphic loci were introgressed. The variation of introgression lines was limited within the diversity between their parents, T. aestivum L. cv. Novosibirskaya 67 (N67) and T. polonicum L. cv. IC12196. N67 was closer to 38 introgression lines than that of IC12196. The UPGMA cluster and principal coordinate analysis (PCO) grouping showed 0.84 to 0.98 similarity values between N67 and the introgression lines. Eleven T. petropavlovskyi accessions were distinguished from introgression lines with UPGMA clusters and PCO groupings, and T. petropavlovskyi was located between the introgressions lines and IC12196. Several introgression lines resembled with T. petropavlovskyi for awning and glume length. The genetic variation among 38 introgression lines was much wider than that of T. petropavlovskyi. We concluded that T. petropavlovskyi was established by intensive selection of hybrid between T. aestivum/T. polonicum.