TEMPORAL-ORDER OF S-PHASE AND MITOSIS IN FISSION YEAST IS DETERMINED BY THE STATE OF THE P34(CDC2) MITOTIC B-CYCLIN COMPLEX
TEMPORAL-ORDER OF S-PHASE AND MITOSIS IN FISSION YEAST IS DETERMINED BY THE STATE OF THE P34(CDC2) MITOTIC B-CYCLIN COMPLEX
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DOI：
10.1016/s0092-8674(94)90542-8
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发表时间：
1994-09-09
期刊：
影响因子：
64.5
通讯作者：
NURSE, P
中科院分区：
文献类型：
--
作者：
HAYLES, J;FISHER, D;NURSE, P
我们发现，p34(Cdc2)-p56(Cdc13)有丝分裂B细胞周期蛋白复合体的状态决定了分裂酵母细胞是经历S期还是有丝分裂。缺失p56(Cdc13)的突变体重置为G1并重新复制其dna，而完全缺乏p34(Cdc2)-p56(Cdc13)复合体的细胞则经历多轮S期。相反，p34(Cdc2)-p56(Cdc13)复合体在G1中的形成不适当地促进细胞进入有丝分裂。我们认为，p34(Cdc2)-p56(Cdc13)复合体的存在或不存在维持了S期和有丝分裂的时间顺序。
We show here that the state of the p34(cdc2)-p56(cdc13) mitotic B cyclin complex determines whether a fission yeast cell undergoes S phase or mitosis. Mutants defective for p56(cdc13) reset to G1 and rereplicate their DNA, while cells completely lacking the p34(cdc2)-p56(cdc13) complex undergo multiple rounds of S phase. In contrast, formation of the p34(cdc2)-p56(cdc13) complex in G1 promotes cells inappropriately into mitosis. We propose that the temporal order of S phase and mitosis is maintained by the presence or absence of the p34(cdc2)-p56(cdc13) complex.