Label-free bond-selective imaging by listening to vibrationally excited molecules.
Label-free bond-selective imaging by listening to vibrationally excited molecules.
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DOI：
10.1103/physrevlett.106.238106
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发表时间：
2011-06-10
影响因子：
8.6
通讯作者：
Cheng JX
中科院分区：
文献类型：
--
作者：
Wang HW;Chai N;Wang P;Hu S;Dou W;Umulis D;Wang LV;Sturek M;Lucht R;Cheng JX
我们报告实现振动光声（VPA）显微镜使用光学激发的分子泛音振动和声学检测所产生的压力瞬变。我们的方法消除了近红外光谱中遇到的组织散射问题，并实现了深度分辨信号收集。CH键在8300 cm−1附近的第二次泛音被激发，此时血液干扰最小。我们展示了富含脂质的动脉粥样硬化斑块的3D VPA成像，通过动脉腔的激发，以及活果蝇幼虫中的脂质储存，具有毫米级的穿透深度。
We report the realization of vibrational photoacoustic (VPA) microscopy using optical excitation of molecular overtone vibration and acoustic detection of the resultant pressure transients. Our approach eliminates the tissue scattering problem encountered in near-infrared spectroscopy and enables depth-resolved signal collection. The 2nd overtone of the CH bond stretch around 8300 cm−1, where blood interference is minimal, is excited. We demonstrate 3D VPA imaging of lipid-rich atherosclerotic plaques by excitation from the artery lumen, and lipid storage in live Drosophila larvae, with millimeter-scale penetration depth.