Biophotonic endoscopy: a review of clinical research techniques for optical imaging and sensing of early gastrointestinal cancer.
Biophotonic endoscopy: a review of clinical research techniques for optical imaging and sensing of early gastrointestinal cancer.
复制标题
生物学内窥镜检查：对早期胃肠道癌的光学成像和传感的临床研究技术的综述。
DOI：
10.1055/s-0034-1392513
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发表时间：
2015-10
影响因子：
2.6
通讯作者：
Thillainayagam AV
中科院分区：
文献类型：
--
作者：
Coda S;Siersema PD;Stamp GW;Thillainayagam AV
检测、定性和分期是胃肠道疾病内窥镜诊断的基本要素，但组织学仍是诊断的金标准。内窥镜技术的新发展可能在不久的将来挑战组织病理学。理想的内窥镜技术应该结合宽视野的“红旗”筛查技术和光学对比度或显微镜方法进行定性和分期，所有这些都在手术过程中同时可用。从理论上讲，生物光子学的进步有可能将这些元素结合起来，从而实现体内的“光学活组织检查”。这些技术最终可能会提高高危病变的发现率和靶向活检和切除的能力，从而减少对活检的需求，降低成本，并减少患者的不确定性。然而，它们在临床实践中的有效性和敏感性必须与传统的组织病理学相对照进行评估。本文介绍了生物光子学在内窥镜光学成像和计量学中的一些最新应用，以及它们的基本原理和作为内窥镜检查工具的临床经验。特别强调了平移无标记光学技术，如荧光光谱、荧光寿命成像显微镜(FLIM)、双光子和多光子显微镜、二次谐波(SHG)和三次谐波(THG)成像、光学相干层析(OCT)、漫反射、拉曼光谱和分子成像。
Detection, characterization, and staging constitute the fundamental elements in the endoscopic diagnosis of gastrointestinal diseases, but histology still remains the diagnostic gold standard. New developments in endoscopic techniques may challenge histopathology in the near future. An ideal endoscopic technique should combine a wide-field, “red flag” screening technique with an optical contrast or microscopy method for characterization and staging, all simultaneously available during the procedure. In theory, biophotonic advances have the potential to unite these elements to allow in vivo “optical biopsy.” These techniques may ultimately offer the potential to increase the rates of detection of high risk lesions and the ability to target biopsies and resections, and so reduce the need for biopsy, costs, and uncertainty for patients. However, their utility and sensitivity in clinical practice must be evaluated against those of conventional histopathology. This review describes some of the most recent applications of biophotonics in endoscopic optical imaging and metrology, along with their fundamental principles and the clinical experience that has been acquired in their deployment as tools for the endoscopist. Particular emphasis has been placed on translational label-free optical techniques, such as fluorescence spectroscopy, fluorescence lifetime imaging microscopy (FLIM), two-photon and multi-photon microscopy, second harmonic generation (SHG) and third harmonic generation (THG) imaging, optical coherence tomography (OCT), diffuse reflectance, Raman spectroscopy, and molecular imaging.