The interaction of two homeobox genes, BREVIPEDICELLUS and PENNYWISE, regulates internode patterning in the Arabidopsis inflorescence
The interaction of two homeobox genes, BREVIPEDICELLUS and PENNYWISE, regulates internode patterning in the Arabidopsis inflorescence
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DOI：
10.1105/tpc.012856
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发表时间：
2003-08-01
期刊：
影响因子：
11.6
通讯作者：
Hake, S
中科院分区：
文献类型：
--
作者：
Smith, HMS;Hake, S
植物的构型是由茎顶端分生组织的活动决定的，茎顶端分生组织分化出叶、节间和腋生分生组织。KNOX基因在植物茎尖分生组织中以特定的模式表达，并在植物结构中起重要作用。KNOX蛋白与BEL1样（BELL）同源结构域蛋白相互作用，并一起以高亲和力结合靶序列。我们已经获得了一个突变的拟南芥钟基因，PENNYWISE（PNY），这似乎表型相似的KNOX突变bravipedicellus（bp）。bp和pny都有随机的较短的节间，并显示腋支的数量略有增加。双突变体显示出一种协同表型，即极短的节间穿插着长的节间和增加的分枝。PNY在花序和花分生组织中表达，并与BP在花序分生组织的离散域中重叠，我们建议节间是图案化的。PNY和BP蛋白的物理关联表明，它们参与了一个复杂的，在花序分生组织中调节早期图案化事件。
Plant architecture results from the activity of the shoot apical meristem, which initiates leaves, internodes, and axillary meristems. KNOTTED1-like homeobox (KNOX) genes are expressed in specific patterns in the shoot apical meristem and play important roles in plant architecture. KNOX proteins interact with BEL1-like (BELL) homeodomain proteins and together bind a target sequence with high affinity. We have obtained a mutation in one of the Arabidopsis BELL genes, PENNYWISE (PNY), that appears phenotypically similar to the KNOX mutant bravipedicellus (bp). Both bp and pny have randomly shorter internodes and display a slight increase in the number of axillary branches. The double mutant shows a synergistic phenotype of extremely short internodes interspersed with long internodes and increased branching. PNY is expressed in inflorescence and floral meristems and overlaps with BP in a discrete domain of the inflorescence meristem where we propose the internode is patterned. The physical association of the PNY and BP proteins suggests that they participate in a complex that regulates early patterning events in the inflorescence meristem.