The cytoskeletal linking proteins, moesin and radixin, are upregulated by platelet-derived growth factor, but not basic fibroblast growth factor in experimental mesangial proliferative glomerulonephritis
The cytoskeletal linking proteins, moesin and radixin, are upregulated by platelet-derived growth factor, but not basic fibroblast growth factor in experimental mesangial proliferative glomerulonephritis
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DOI：
10.1172/jci118697
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发表时间：
1996-06-01
影响因子：
15.9
通讯作者：
Johnson, RJ
中科院分区：
文献类型：
--
作者：
Hugo, C;Hugo, C;Johnson, RJ
在大鼠实验性系膜增生性肾炎（抗 Thy1 模型）中检查了两种细胞骨架连接蛋白（moesin 和 radixin）的表达。 Moesin 和 radixin mRNA 和蛋白质在正常大鼠肾小球的所有细胞类型（足细胞除外）中组成型表达。在抗Thy1模型中，浸润巨噬细胞和活化的α平滑肌肌动蛋白阳性系膜细胞中moesin和radixin的表达增加，并且集中在肾小球重塑区域的系膜细胞的细胞延伸中。使用中和抗体的研究表明，系膜细胞中 moesin 和 radixin 表达的增加是由 PDGF 介导的，而不是 bFGF 介导的。这些细胞骨架蛋白的增加似乎主要（radixin）或部分（moesin）在转录后受到调节。数据表明，PDGF 介导的细胞骨架蛋白、moesin 和 radixin 的上调对于细胞迁移和损伤后伴随肾小球结构协调恢复的其他变化非常重要。
The expression of the two cytoskeletal linking proteins, moesin and radixin, was examined in experimental mesangial proliferative nephritis in rats (anti-Thy1 model). Moesin and radixin mRNA and protein are constitutively expressed in all cell types of normal rat glomeruli, except podocytes. In the anti-Thy1 model the expression of moesin and radixin was increased in infiltrating macrophages and in activated, alpha-smooth muscle actin-positive mesangial cells and was concentrated in the cellular extensions of mesangial cells in areas of glomerular remodelling. Studies using neutralizing antibodies demonstrated that the increase in moesin and radixin expression by mesangial cells is mediated by PDGF, but not bFGF. The increase in these cytoskeletal proteins appears to be regulated primarily (radixin) or partially (moesin) posttranscriptionally. The data suggest that PDGF mediated upregulation of the cytoskeletal proteins, moesin and radixin, is important for cell migration and other changes that accompany the coordinated restoration of glomerular architecture after injury.