Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
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DOI：
10.1016/j.cbpb.2005.05.044
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发表时间：
2005-06-01
影响因子：
2.3
通讯作者：
Bai, JF
中科院分区：
文献类型：
--
作者：
Mahroof, R;Zhu, KY;Bai, JF
在castaneum （Tribolium castaneum, Herbst）中鉴定出3个编码热休克蛋白70’s的基因，暂定为tchsp70 I、tchsc70 II和tchsp70 III。结果表明，tcsp70 I和tcsc70 II的氨基酸序列同源性为99%。然而，tchsp70 III的氨基酸序列与tchsp70 I和tchsc70 II的相同度仅为58.5%。在23℃（对照）和40℃（热休克）条件下分别处理1 h的木纹螟幼虫、老虫、蛹和成虫中，研究了tchsp70的分期特异性表达模式。采用Northern blot和实时定量PCR检测各组mRNA水平。通过Northern blotting检测这三个基因的转录本，tchsp70 1、tchsc70 H和tchsp70 III的转录本大小分别为2.4 ~ 2.2 kb和2.3 kb。在热休克发育阶段，与对照相比，tchsp70 mRNA的表达增加了1.1- 2.0倍。热休克昆虫和对照昆虫的tchsp70 II mRNA在不同发育阶段的表达相似，而tchsp70 X mRNA在不同发育阶段的表达不同。结果表明，tchsp70i基因是热诱导型的，tchsp70ii基因是组成型的，而tchsp70x基因是受发育调控的。(C) 2005爱思唯尔公司版权所有。
Three genes were identified encoding heat shock protein 70's in Tribolium castaneum (Herbst) and they were tentatively named as tchsp70 I, tchsc70 II, and tchsp70 III. Comparison of deduced amino acid sequences of tchsp70 I and tchsc70 II showed 99% identity. However, the amino acid sequence of tchsp70 III was only 58.5% identical to those of tchsp70 I and tchsc70 II. Stage-specific expression patterns of the tchsp70 were investigated in young larvae, old larvae, pupae, and adults of T castaneum exposed for 1 h to 23 degrees C (control) or 40 degrees C (heat-shock). Northern blot and real-time quantitative PCR analyses were carried out to determine mRNA levels in each life stage. Transcripts of all three genes were detected by Northern blotting, and the sizes were 2.4- 2.2-, and 2.3-kb for tchsp70 1, tchsc70 H, and tchsp70 III, respectively. A 1.1- to 2.0-fold increased expression of tchsp70 I mRNA was found in heat-shocked developmental stages compared with the control. The expression of tchsp70 II mRNA among developmental stages was similar between heat-shocked and control insects, and the expression of tchsp70 X mRNA varied among developmental stages. Results suggest that the expression of tchsp70 I gene is heat-inducible, tchsc70 II is constitutive, and tchsp70 X is developmentally regulated in T castaneum. (C) 2005 Elsevier Inc. All rights reserved.