Partial characterization of a mitogenic factor with somatomedin‐like activity produced by culture WI‐38 human fibroblasts
Partial characterization of a mitogenic factor with somatomedin‐like activity produced by culture WI‐38 human fibroblasts
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培养 WI-38 人成纤维细胞产生的具有生长调节素样活性的促有丝分裂因子的部分表征
DOI：
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发表时间：
1981
影响因子：
5.6
通讯作者：
R. Bala
中科院分区：
文献类型：
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作者：
P. Atkison;R. Bala
发现从WI-38人成纤维细胞获得的条件无血清培养基（CSFM）含有具有生长调节蛋白（SM）样活性的促有丝分裂因子。用放线菌酮处理细胞消除了CSFM中的SM样活性，表明这些细胞产生并释放活性。凝胶过滤显示成纤维细胞SM样活性（FSLA）的分子大小接近45，000。该FSLA的等电聚焦产生2条SM活性带，pI为4.7和6.1，相应的分子大小分别为1629，000和16，500。通过凝胶过滤获得的FSLA显示了SM放射受体和放射免疫测定中与基本SM平行的剂量反应曲线，并刺激：（1）垂体切除大鼠软骨的35 SO 4摄取;（2）分离大鼠脂肪细胞的（U-14 C）葡萄糖氧化;以及（3）这些相同WI-38成纤维细胞的（3 H）胸苷摄取和细胞分裂。我们的研究表明，这种FSLA和基本SM是相似的，但不完全相同。
Conditioned serum‐free medium (CSFM) obtained from WI‐38 human fibroblasts was found to contain a mitogenic factor(s) with somatomedin (SM)‐like activity. Treatment of the cells with cycloheximide eliminated the SM‐like activity in CSFM, suggesting that these cells produce and release the activity. Gel filtration revealed that the fibroblast SM‐like activity (FSLA) had a molecular size near 45,000. Isoelectric focusing of this FSLA yielded 2 bands of SM activity with pIs of 4.7 and 6.1, and corresponding molecular sizes of ∼29,000 and 16,500, respectively. The FSLA obtained by gel filtration revealed parallel dose response curves with a basic SM in a SM radioreceptor and radioimmunoassay and stimulated: (1) 35So4 uptake by hypophysectomized rat cartilage; (2) (U‐14C) glucose oxidation is isolated rat adipocytes; and (3) (3H) thymidine uptake and cell division in these same WI‐38 fibroblasts. Out studies indicate that this FSLA and basic SM are similar but not identical.